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Article

The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis

1
Laboratorio de Virología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo 39070, Mexico
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Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana Unidad Cuajimalpa, Mexico City 05300, Mexico
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Laboratorio de Epigenética del Cáncer, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo 39070, Mexico
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Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo 39070, Mexico
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CONACyT-Universidad Autónoma de Guerrero, Chilpancingo 39070, Mexico
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Laboratorio de Citopatología e Histoquímica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo 39070, Mexico
*
Author to whom correspondence should be addressed.
Academic Editors: Thomas Iftner and Frank Stubenrauch
Viruses 2022, 14(5), 963; https://doi.org/10.3390/v14050963
Received: 6 April 2022 / Revised: 30 April 2022 / Accepted: 3 May 2022 / Published: 5 May 2022
(This article belongs to the Special Issue Recent Advances in Papillomaviruses Research)
The E6 oncoprotein of HPV16 variants differentially alters the transcription of the genes involved in migration and non-coding RNAs such as lncRNAs. The role of the lncRNA MINCR in cervical cancer and its relationship with variants of oncogenic HPV remain unknown. Therefore, the objective of this study was to analyze the effect of the E6 oncoprotein of the AA-c variant of HPV16 in cell migration through the MINCR/miR-28-5p/RAP1B axis. To explore the functional role of MINCR in CC, we used an in vitro model of C33-A cells with exogenous expression of the E6 oncoprotein of the AA-c variant of HPV16. Interfering RNAs performed MINCR silencing, and the expression of miR-28-5p and RAP1B mRNA was analyzed by RT-qPCR. We found that C33-A/AA-c cells expressed MINCR 8-fold higher compared to the control cells. There is an inverse correlation between the expression of miR-28-5p and RAP1B in C33-A/AA-c cells. Our results suggest that MINCR might regulate the expression of RAP1B through the inhibition of miR-28-5p in CC cells expressing the E6 oncoprotein of HPV16 AA-c. We report, for the first time, that the MINCR/miR-28-5p/RAP1B axis positively regulates cell migration in CC-derived cells that express the E6 oncoprotein of the AA-c variant of HPV16. View Full-Text
Keywords: lncRNAs; MINCR; miR-28-5p; RAP1B; E6 oncoprotein; HPV16 AA-c variant lncRNAs; MINCR; miR-28-5p; RAP1B; E6 oncoprotein; HPV16 AA-c variant
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MDPI and ACS Style

Perez-Bacho, E.G.; Beltrán-Anaya, F.O.; Arechaga-Ocampo, E.; Hernández-Sotelo, D.; Garibay-Cerdenares, O.L.; Illades-Aguiar, B.; Alarcón-Romero, L.D.C.; Del Moral-Hernández, O. The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis. Viruses 2022, 14, 963. https://doi.org/10.3390/v14050963

AMA Style

Perez-Bacho EG, Beltrán-Anaya FO, Arechaga-Ocampo E, Hernández-Sotelo D, Garibay-Cerdenares OL, Illades-Aguiar B, Alarcón-Romero LDC, Del Moral-Hernández O. The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis. Viruses. 2022; 14(5):963. https://doi.org/10.3390/v14050963

Chicago/Turabian Style

Perez-Bacho, Eduardo Gil, Fredy Omar Beltrán-Anaya, Elena Arechaga-Ocampo, Daniel Hernández-Sotelo, Olga Lilia Garibay-Cerdenares, Berenice Illades-Aguiar, Luz Del Carmen Alarcón-Romero, and Oscar Del Moral-Hernández. 2022. "The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis" Viruses 14, no. 5: 963. https://doi.org/10.3390/v14050963

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