Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. HAdV26 Infection Triggers Expression of Pro-Inflammatory Cytokines in Human Epithelial Cells In Vitro
3.2. HAdV26 Triggered IL-6 Gene Expression Is αvβ3 Integrin Dependent
3.3. HAdV26-Induced IL-6 Gene Expression Is NF-κB Mediated
3.4. HAdV26-Induced Production of IL-6 Is αvβ3 Integrin Dependent
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene ID | Primer Sequences |
---|---|
IL-6 | F: 5′CAATGAGGAGACTTGCCTGG3′ R: 5′GCACAGCTCTGGCTTGTTCC3′ |
IL-8 | F: 5′GTTTTTGAAGAGGGCTGAGAATTC3′ R: 5′ATGAAGTGTTGAAGTAGATTTGCTTG3′ |
IL-1β | F: 5′TGGCAATGAGGATGACTTGTTC3′ R: 5′CTGTAGTGGTGGTCGGAGATT3′ |
TNF-α | F: 5′AACCTCCTCTCTGCCATCAA3′ R: 5′GGAAGACCCCTCCCAGATAG3′ |
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Nestić, D.; Božinović, K.; Drašković, I.; Kovačević, A.; van den Bosch, J.; Knežević, J.; Custers, J.; Ambriović-Ristov, A.; Majhen, D. Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner. Viruses 2022, 14, 672. https://doi.org/10.3390/v14040672
Nestić D, Božinović K, Drašković I, Kovačević A, van den Bosch J, Knežević J, Custers J, Ambriović-Ristov A, Majhen D. Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner. Viruses. 2022; 14(4):672. https://doi.org/10.3390/v14040672
Chicago/Turabian StyleNestić, Davor, Ksenija Božinović, Isabela Drašković, Alen Kovačević, Jolien van den Bosch, Jelena Knežević, Jerome Custers, Andreja Ambriović-Ristov, and Dragomira Majhen. 2022. "Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner" Viruses 14, no. 4: 672. https://doi.org/10.3390/v14040672