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Article

Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner

1
Laboratory for Cell Biology and Signalling, Division of Molecular Biology, Ruđer Bošković Institute, 10000 Zagreb, Croatia
2
Laboratory for Advanced Genomics, Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia
3
Faculty for Dental Medicine and Health, University of Osijek, 31000 Osijek, Croatia
4
Janssen Vaccines and Preventions BV, 2333 CA Leiden, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Akinori Takaoka
Viruses 2022, 14(4), 672; https://doi.org/10.3390/v14040672
Received: 11 February 2022 / Revised: 16 March 2022 / Accepted: 22 March 2022 / Published: 24 March 2022
(This article belongs to the Special Issue State-of-the-Art Virology Research in Croatia 2022)
The low seroprevalent human adenovirus type 26 (HAdV26)-based vaccine vector was the first adenovirus-based vector to receive marketing authorization from European Commission. HAdV26-based vaccine vectors induce durable humoral and cellular immune responses and, as such, represent a highly valuable tool for fighting infectious diseases. Despite well-described immunogenicity in vivo, the basic biology of HAdV26 still needs some refinement. The aim of this study was to determine the pro-inflammatory cytokine profile of epithelial cells infected with HAdV26 and then investigate the underlying molecular mechanism. The expression of studied genes and proteins was assessed by quantitative polymerase chain reaction, western blot, and enzyme-linked immunosorbent assay. Confocal microscopy was used to visualize HAdV26 cell uptake. We found that HAdV26 infection in human epithelial cells triggers the expression of pro-inflammatory cytokines and chemokines, namely IL-6, IL-8, IL-1β, and TNF-α, with the most pronounced difference shown for IL-6. We investigated the underlying molecular mechanism and observed that HAdV26-induced IL-6 gene expression is αvβ3 integrin dependent and NF-κB mediated. Our findings provide new data regarding pro-inflammatory cytokine and chemokine expression in HAdV26-infected epithelial cells, as well as details concerning HAdV26-induced host signaling pathways. Information obtained within this research increases our current knowledge of HAdV26 basic biology and, as such, can contribute to further development of HAdV26-based vaccine vectors. View Full-Text
Keywords: human adenovirus type 26; vaccine vector; innate immune response; integrin αvβ3 human adenovirus type 26; vaccine vector; innate immune response; integrin αvβ3
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MDPI and ACS Style

Nestić, D.; Božinović, K.; Drašković, I.; Kovačević, A.; van den Bosch, J.; Knežević, J.; Custers, J.; Ambriović-Ristov, A.; Majhen, D. Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner. Viruses 2022, 14, 672. https://doi.org/10.3390/v14040672

AMA Style

Nestić D, Božinović K, Drašković I, Kovačević A, van den Bosch J, Knežević J, Custers J, Ambriović-Ristov A, Majhen D. Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner. Viruses. 2022; 14(4):672. https://doi.org/10.3390/v14040672

Chicago/Turabian Style

Nestić, Davor, Ksenija Božinović, Isabela Drašković, Alen Kovačević, Jolien van den Bosch, Jelena Knežević, Jerome Custers, Andreja Ambriović-Ristov, and Dragomira Majhen. 2022. "Human Adenovirus Type 26 Induced IL-6 Gene Expression in an αvβ3 Integrin- and NF-κB-Dependent Manner" Viruses 14, no. 4: 672. https://doi.org/10.3390/v14040672

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