Next Article in Journal
Viral Aggregation: The Knowns and Unknowns
Previous Article in Journal
V5 and GFP Tagging of Viral Gene pp38 of Marek’s Disease Vaccine Strain CVI988 Using CRISPR/Cas9 Editing
Previous Article in Special Issue
In Silico Prediction and Selection of Target Sequences in the SARS-CoV-2 RNA Genome for an Antiviral Attack
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 14
Issue 2
10.3390/v14020437
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus

by
Daria Prokhorova
1,2,
Natalya Zhukova (Eschenko)
1,
Anna Lemza
1,
Mariia Sergeeva
1,3,
Rinat Amirkhanov
1 and
Grigory Stepanov
1,*
1
Laboratory of Genome Editing, Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
2
Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
3
Laboratory of Vector Vaccines, Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation, 197376 Saint Petersburg, Russia
*
Author to whom correspondence should be addressed.
Viruses 2022, 14(2), 437; https://doi.org/10.3390/v14020437
Submission received: 28 December 2021 / Revised: 4 February 2022 / Accepted: 16 February 2022 / Published: 21 February 2022
(This article belongs to the Special Issue CRISPR/Cas in Viral Research)
Browse Figure
Versions Notes

Abstract

Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.
Keywords: CRISPR/Cas9; influenza virus; cellular immune response; cell receptors; RNA processing factors; CRISPR/Cas9 genome screening CRISPR/Cas9; influenza virus; cellular immune response; cell receptors; RNA processing factors; CRISPR/Cas9 genome screening

Share and Cite

MDPI and ACS Style

Prokhorova, D.; Zhukova, N.; Lemza, A.; Sergeeva, M.; Amirkhanov, R.; Stepanov, G. Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus. Viruses 2022, 14, 437. https://doi.org/10.3390/v14020437

AMA Style

Prokhorova D, Zhukova N, Lemza A, Sergeeva M, Amirkhanov R, Stepanov G. Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus. Viruses. 2022; 14(2):437. https://doi.org/10.3390/v14020437

Chicago/Turabian Style

Prokhorova, Daria, Natalya Zhukova (Eschenko), Anna Lemza, Mariia Sergeeva, Rinat Amirkhanov, and Grigory Stepanov. 2022. "Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus" Viruses 14, no. 2: 437. https://doi.org/10.3390/v14020437

APA Style

Prokhorova, D., Zhukova, N., Lemza, A., Sergeeva, M., Amirkhanov, R., & Stepanov, G. (2022). Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus. Viruses, 14(2), 437. https://doi.org/10.3390/v14020437

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop