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Volume 14
Issue 2
10.3390/v14020222
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Article

Functional Characterization of Replication-Associated Proteins Encoded by Alphasatellites Identified in Yunnan Province, China

by
Liling Zhao
1,2,
Xuan Che
1,
Zhanqi Wang
3,
Xueping Zhou
1,4 and
Yan Xie
1,*
1
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
2
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
3
Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou 313000, China
4
Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
*
Author to whom correspondence should be addressed.
Viruses 2022, 14(2), 222; https://doi.org/10.3390/v14020222
Submission received: 22 December 2021 / Revised: 17 January 2022 / Accepted: 21 January 2022 / Published: 24 January 2022
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

Alphasatellites, which encode only a replication-associated protein (alpha-Rep), are frequently found to be non-essential satellite components associated with begomovirus/betasatellite complexes, and their presence can modulate disease symptoms and/or viral DNA accumulation during infection. Our previous study has shown that there are three types of alphasatellites associated with begomovirus/betasatellite complexes in Yunnan province in China and they encode three corresponding types of alpha-Rep proteins. However, the biological functions of alpha-Reps remain poorly understood. In this study, we investigated the biological functions of alpha-Reps in post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS) using 16c and 16-TGS transgenic Nicotiana benthamiana plants. Results showed that all the three types of alpha-Rep proteins were capable of suppressing the PTGS and reversing the TGS. Among them, the alpha-Rep of Y10DNA1 has the strongest PTGS and TGS suppressor activities. We also found that the alpha-Rep proteins were able to increase the accumulation of their helper virus during coinfection. These results suggest that the alpha-Reps may have a role in overcoming host defense, which provides a possible explanation for the selective advantage provided by the association of alphasatellites with begomovirus/betasatellite complexes.
Keywords: alphasatellite; replication-associated protein; geminivirus complex; PTGS; TGS alphasatellite; replication-associated protein; geminivirus complex; PTGS; TGS

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MDPI and ACS Style

Zhao, L.; Che, X.; Wang, Z.; Zhou, X.; Xie, Y. Functional Characterization of Replication-Associated Proteins Encoded by Alphasatellites Identified in Yunnan Province, China. Viruses 2022, 14, 222. https://doi.org/10.3390/v14020222

AMA Style

Zhao L, Che X, Wang Z, Zhou X, Xie Y. Functional Characterization of Replication-Associated Proteins Encoded by Alphasatellites Identified in Yunnan Province, China. Viruses. 2022; 14(2):222. https://doi.org/10.3390/v14020222

Chicago/Turabian Style

Zhao, Liling, Xuan Che, Zhanqi Wang, Xueping Zhou, and Yan Xie. 2022. "Functional Characterization of Replication-Associated Proteins Encoded by Alphasatellites Identified in Yunnan Province, China" Viruses 14, no. 2: 222. https://doi.org/10.3390/v14020222

APA Style

Zhao, L., Che, X., Wang, Z., Zhou, X., & Xie, Y. (2022). Functional Characterization of Replication-Associated Proteins Encoded by Alphasatellites Identified in Yunnan Province, China. Viruses, 14(2), 222. https://doi.org/10.3390/v14020222

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