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Article

A Duplex Fluorescent Microsphere Immunoassay for Detection of Bluetongue and Epizootic Hemorrhagic Disease Virus Antibodies in Cattle Sera

Center for Grain and Animal Health, Agricultural Research Service, Arthropod-Borne Animal Diseases Research Unit, United States Department of Agriculture, Manhattan, KS 66502, USA
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Author to whom correspondence should be addressed.
Academic Editor: Samantha M. Wisely
Viruses 2021, 13(4), 682; https://doi.org/10.3390/v13040682
Received: 31 March 2021 / Revised: 10 April 2021 / Accepted: 14 April 2021 / Published: 15 April 2021
(This article belongs to the Special Issue Epizootic Hemorrhagic Disease Virus)
Bluetongue virus (BTV) causes internationally reportable hemorrhagic disease in cattle, sheep, and white-tailed deer. The closely related, and often co-circulating, epizootic hemorrhagic disease virus causes a clinically similar devastating disease in white-tailed deer, with increasing levels of disease in cattle in the past 10 years. Transmitted by Culicoides biting midges, together, they constitute constant disease threats to the livelihood of livestock owners. In cattle, serious economic impacts result from decreased animal production, but most significantly from trade regulations. For effective disease surveillance and accurate trade regulation implementation, rapid, sensitive assays that can detect exposure of cattle to BTV and/or EHDV are needed. We describe the development and validation of a duplex fluorescent microsphere immunoassay (FMIA) to simultaneously detect and differentiate antibodies to BTV and EHDV in a single bovine serum sample. Performance of the duplex FMIA for detection and differentiation of BTV and EHDV serogroup antibodies was comparable, with higher sensitivity than commercially available single-plex competitive enzyme-linked immunosorbent assays (cELISA) for detection of each virus antibody separately. The FMIA adds to the currently available diagnostic tools for hemorrhagic orbiviral diseases in cattle as a sensitive, specific assay, with the benefits of serogroup differentiation in a single serum sample, and multiplexing flexibility in a high-throughput platform. View Full-Text
Keywords: fluorescent microsphere immunoassay; FMIA; bluetongue virus; epizootic hemorrhagic disease virus; cattle; antibody; serology fluorescent microsphere immunoassay; FMIA; bluetongue virus; epizootic hemorrhagic disease virus; cattle; antibody; serology
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MDPI and ACS Style

Drolet, B.S.; Reister-Hendricks, L.M. A Duplex Fluorescent Microsphere Immunoassay for Detection of Bluetongue and Epizootic Hemorrhagic Disease Virus Antibodies in Cattle Sera. Viruses 2021, 13, 682. https://doi.org/10.3390/v13040682

AMA Style

Drolet BS, Reister-Hendricks LM. A Duplex Fluorescent Microsphere Immunoassay for Detection of Bluetongue and Epizootic Hemorrhagic Disease Virus Antibodies in Cattle Sera. Viruses. 2021; 13(4):682. https://doi.org/10.3390/v13040682

Chicago/Turabian Style

Drolet, Barbara S., and Lindsey M. Reister-Hendricks 2021. "A Duplex Fluorescent Microsphere Immunoassay for Detection of Bluetongue and Epizootic Hemorrhagic Disease Virus Antibodies in Cattle Sera" Viruses 13, no. 4: 682. https://doi.org/10.3390/v13040682

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