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Volume 13
Issue 4
10.3390/v13040591
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Article

Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases

by
Ryota Sasaki
1,
Shuhei Miyashita
1,
Sugihiro Ando
1,
Kumiko Ito
1,
Toshiyuki Fukuhara
2 and
Hideki Takahashi
1,*
1
Graduate School of Agricultural Science, Tohoku University, 468-1, Aramaki-Aza-Aoba, Sendai 980-0845, Japan
2
Department of Applied Biological Sciences and Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8538, Japan
*
Author to whom correspondence should be addressed.
Viruses 2021, 13(4), 591; https://doi.org/10.3390/v13040591
Submission received: 8 March 2021 / Revised: 26 March 2021 / Accepted: 27 March 2021 / Published: 31 March 2021
(This article belongs to the Special Issue Bacteriophage-Based Biocontrol in Agriculture)
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Abstract

Jumbo phages have DNA genomes larger than 200 kbp in large virions composed of an icosahedral head, tail, and other adsorption structures, and they are known to be abundant biological substances in nature. In this study, phages in leaf litter compost were screened for their potential to suppress rice seedling rot disease caused by the bacterium Burkholderia glumae, and a novel phage was identified in a filtrate-enriched suspension of leaf litter compost. The phage particles consisted of a rigid tailed icosahedral head and contained a DNA genome of 227,105 bp. The phage could lyse five strains of B. glumae and six strains of Burkholderia plantarii. The phage was named jumbo Burkholderia phage FLC6. Proteomic tree analysis revealed that phage FLC6 belongs to the same clade as two jumbo Ralstonia phages, namely RSF1 and RSL2, which are members of the genus Chiangmaivirus (family: Myoviridae; order: Caudovirales). Interestingly, FLC6 could also lyse two strains of Ralstonia pseudosolanacearum, the causal agent of bacterial wilt, suggesting that FLC6 has a broad host range that may make it especially advantageous as a bio-control agent for several bacterial diseases in economically important crops. The novel jumbo phage FLC6 may enable leaf litter compost to suppress several bacterial diseases and may itself be useful for controlling plant diseases in crop cultivation.
Keywords: biocontrol agent; broad host range; leaf litter compost; Burkholderia; Ralstonia; jumbo phage; phage therapy biocontrol agent; broad host range; leaf litter compost; Burkholderia; Ralstonia; jumbo phage; phage therapy

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MDPI and ACS Style

Sasaki, R.; Miyashita, S.; Ando, S.; Ito, K.; Fukuhara, T.; Takahashi, H. Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases. Viruses 2021, 13, 591. https://doi.org/10.3390/v13040591

AMA Style

Sasaki R, Miyashita S, Ando S, Ito K, Fukuhara T, Takahashi H. Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases. Viruses. 2021; 13(4):591. https://doi.org/10.3390/v13040591

Chicago/Turabian Style

Sasaki, Ryota, Shuhei Miyashita, Sugihiro Ando, Kumiko Ito, Toshiyuki Fukuhara, and Hideki Takahashi. 2021. "Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases" Viruses 13, no. 4: 591. https://doi.org/10.3390/v13040591

APA Style

Sasaki, R., Miyashita, S., Ando, S., Ito, K., Fukuhara, T., & Takahashi, H. (2021). Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases. Viruses, 13(4), 591. https://doi.org/10.3390/v13040591

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