Next Article in Journal
Non-Polio Enteroviruses from Acute Flaccid Paralysis Surveillance in Korea, 2012–2019
Next Article in Special Issue
Spatial Analysis of the 2017 Outbreak of Hemorrhagic Disease and Physiographic Region in the Eastern United States
Previous Article in Journal
Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection
Previous Article in Special Issue
Tracking Community Timing: Pattern and Determinants of Seasonality in Culicoides (Diptera: Ceratopogonidae) in Northern Florida
Article

Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2

1
Center for Grain and Animal Health Research, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS 66502, USA
2
Florida Medical Entomology Laboratory, Institute of Food and Agricultural Sciences, University of Florida, Vero Beach, FL 32962, USA
3
Department of Environmental & Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USA
4
Department of Wildlife Ecology and Conservation, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Shan-Lu Liu
Viruses 2021, 13(3), 410; https://doi.org/10.3390/v13030410
Received: 12 February 2021 / Revised: 3 March 2021 / Accepted: 4 March 2021 / Published: 5 March 2021
(This article belongs to the Special Issue Epizootic Hemorrhagic Disease Virus)
Epizootic hemorrhagic disease virus (EHDV; family Reoviridae, genus Orbivirus) is an arthropod-borne virus of ungulates, primarily white-tailed deer in North America. Culicoides sonorensis, the only confirmed North American vector of EHDV, is rarely collected from Florida despite annual virus outbreaks. Culicoides insignis is an abundant species in Florida and is also a confirmed vector of the closely related Bluetongue virus. In this study, oral challenge of C. insignis was performed to determine vector competence for EHDV serotype-2. Field-collected female midges were provided bovine blood spiked with three different titers of EHDV-2 (5.05, 4.00, or 2.94 log10PFUe/mL). After an incubation period of 10 days or after death, bodies and legs were collected. Saliva was collected daily from all females from 3 days post feeding until their death using honey card assays. All samples were tested for EHDV RNA using RT-qPCR. Our results suggest that C. insignis is a weakly competent vector of EHDV-2 that can support a transmissible infection when it ingests a high virus titer (29% of midges had virus positive saliva when infected at 5.05 log10PFUe/mL), but not lower virus titers. Nevertheless, due to the high density of this species, particularly in peninsular Florida, it is likely that C. insignis plays a role in the transmission of EHDV-2. View Full-Text
Keywords: arboviruses; Reoviridae; Orbivirus arboviruses; Reoviridae; Orbivirus
Show Figures

Graphical abstract

MDPI and ACS Style

McGregor, B.L.; Erram, D.; Alto, B.W.; Lednicky, J.A.; Wisely, S.M.; Burkett-Cadena, N.D. Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2. Viruses 2021, 13, 410. https://doi.org/10.3390/v13030410

AMA Style

McGregor BL, Erram D, Alto BW, Lednicky JA, Wisely SM, Burkett-Cadena ND. Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2. Viruses. 2021; 13(3):410. https://doi.org/10.3390/v13030410

Chicago/Turabian Style

McGregor, Bethany L., Dinesh Erram, Barry W. Alto, John A. Lednicky, Samantha M. Wisely, and Nathan D. Burkett-Cadena 2021. "Vector Competence of Florida Culicoides insignis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype-2" Viruses 13, no. 3: 410. https://doi.org/10.3390/v13030410

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop