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Volume 13
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Article

Expression of an Antiviral Gene GmRUN1 from Soybean Is Regulated via Intron-Mediated Enhancement (IME)

by
Pengfei Diao
1,†,
Hongyu Sun
1,†,
Zhuo Bao
1,
Wenxia Li
1,
Niu Niu
1,
Weimin Li
2 and
Hada Wuriyanghan
1,*
1
Key Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China
2
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Viruses 2021, 13(10), 2032; https://doi.org/10.3390/v13102032
Submission received: 31 August 2021 / Revised: 28 September 2021 / Accepted: 29 September 2021 / Published: 8 October 2021
(This article belongs to the Special Issue State-of-the-Art Plant-Virus Interactions in Asia)
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Abstract

Most of R (resistance) genes encode the protein containing NBS-LRR (nucleotide binding site and leucine-rich repeat) domains. Here, N. benthamiana plants were used for transient expression assays at 3–4 weeks of age. We identified a TNL (TIR-NBS-LRR) encoding gene GmRUN1 that was resistant to both soybean mosaic virus (SMV) and tobacco mosaic virus (TMV). Truncation analysis indicated the importance of all three canonical domains for GmRUN1-mediated antiviral activity. Promoter-GUS analysis showed that GmRUN1 expression is inducible by both salicylic acid (SA) and a transcription factor GmDREB3 via the cis-elements as-1 and ERE (ethylene response element), which are present in its promoter region. Interestingly, GmRUN1 gDNA (genomic DNA) shows higher viral resistance than its cDNA (complementary DNA), indicating the existence of intron-mediated enhancement (IME) for GmRUN1 regulation. We provided evidence that intron2 of GmRUN1 increased the mRNA level of native gene GmRUN1, a soybean antiviral gene SRC7 and also a reporter gene Luciferase, indicating the general transcriptional enhancement of intron2 in different genes. In summary, we identified an antiviral TNL type soybean gene GmRUN1, expression of which was regulated at different layers. The investigation of GmRUN1 gene regulatory network would help to explore the mechanism underlying soybean-SMV interactions.
Keywords: GmDREB3; GmRUN1; intron-mediated enhancement; resistance gene; salicylic acid; soybean mosaic virus; tobacco mosaic virus; transcriptional regulation GmDREB3; GmRUN1; intron-mediated enhancement; resistance gene; salicylic acid; soybean mosaic virus; tobacco mosaic virus; transcriptional regulation

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MDPI and ACS Style

Diao, P.; Sun, H.; Bao, Z.; Li, W.; Niu, N.; Li, W.; Wuriyanghan, H. Expression of an Antiviral Gene GmRUN1 from Soybean Is Regulated via Intron-Mediated Enhancement (IME). Viruses 2021, 13, 2032. https://doi.org/10.3390/v13102032

AMA Style

Diao P, Sun H, Bao Z, Li W, Niu N, Li W, Wuriyanghan H. Expression of an Antiviral Gene GmRUN1 from Soybean Is Regulated via Intron-Mediated Enhancement (IME). Viruses. 2021; 13(10):2032. https://doi.org/10.3390/v13102032

Chicago/Turabian Style

Diao, Pengfei, Hongyu Sun, Zhuo Bao, Wenxia Li, Niu Niu, Weimin Li, and Hada Wuriyanghan. 2021. "Expression of an Antiviral Gene GmRUN1 from Soybean Is Regulated via Intron-Mediated Enhancement (IME)" Viruses 13, no. 10: 2032. https://doi.org/10.3390/v13102032

APA Style

Diao, P., Sun, H., Bao, Z., Li, W., Niu, N., Li, W., & Wuriyanghan, H. (2021). Expression of an Antiviral Gene GmRUN1 from Soybean Is Regulated via Intron-Mediated Enhancement (IME). Viruses, 13(10), 2032. https://doi.org/10.3390/v13102032

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