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Article

Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus

1
School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK
2
Department of Microbiology, College of Medicine, Thi-Qar University, Thi-Qar, Iraq
3
Biology Department, Texas A&M University, Texarkana, TX 75503, USA
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(9), 1054; https://doi.org/10.3390/v12091054
Received: 20 August 2020 / Revised: 16 September 2020 / Accepted: 17 September 2020 / Published: 22 September 2020
(This article belongs to the Collection Coronaviruses)
Coronaviruses (CoVs) are enveloped, positive sense, single strand RNA viruses that cause respiratory, intestinal and neurological diseases in mammals and birds. Following replication, CoVs assemble on intracellular membranes including the endoplasmic reticulum Golgi intermediate compartment (ERGIC) where the envelope protein (E) functions in virus assembly and release. In consequence, E potentially contains membrane-modifying peptides. To search for such peptides, the E coding sequence of Mouse Hepatitis Virus (MHV) was inspected for its amino acid conservation, proximity to the membrane and/or predicted amphipathic helices. Peptides identified in silico were synthesized and tested for membrane-modifying activity in the presence of giant unilamellar vesicles (GUVs) consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), sphingomyelin and cholesterol. To confirm the presence of membrane binding peptides identified in the context of a full-length E protein, the wild type and a number of mutants in the putative membrane binding peptide were expressed in Lenti-X-293T mammalian and insect cells, and the distribution of E antigen within the expressing cell was assessed. Our data identify a role for the post-transmembrane region of MHV E in membrane binding. View Full-Text
Keywords: coronavirus; envelope; E protein; peptide; membrane; GUV; expression coronavirus; envelope; E protein; peptide; membrane; GUV; expression
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MDPI and ACS Style

Alsaadi, E.A.J.; Neuman, B.W.; Jones, I.M. Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus. Viruses 2020, 12, 1054. https://doi.org/10.3390/v12091054

AMA Style

Alsaadi EAJ, Neuman BW, Jones IM. Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus. Viruses. 2020; 12(9):1054. https://doi.org/10.3390/v12091054

Chicago/Turabian Style

Alsaadi, Entedar A.J., Benjamin W. Neuman, and Ian M. Jones 2020. "Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus" Viruses 12, no. 9: 1054. https://doi.org/10.3390/v12091054

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