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Article

Mapping Attenuation Determinants in Enterovirus-D68

1
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA
2
Industrial and Applied Genomics, AI and Cognitive Software, IBM Almaden Research Center, San Jose, CA 95120, USA
3
Center for Cellular Construction, University of California, San Francisco, CA 94158, USA
4
Graduate Group in Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(8), 867; https://doi.org/10.3390/v12080867
Received: 10 July 2020 / Revised: 5 August 2020 / Accepted: 5 August 2020 / Published: 8 August 2020
(This article belongs to the Special Issue Enteroviruses 2021)
Enterovirus (EV)-D68 has been associated with epidemics in the United Sates in 2014, 2016 and 2018. This study aims to identify potential viral virulence determinants. We found that neonatal type I interferon receptor knockout mice are susceptible to EV-D68 infection via intraperitoneal inoculation and were able to recapitulate the paralysis process observed in human disease. Among the EV-D68 strains tested, strain US/MO-14-18949 caused no observable disease in this mouse model, whereas the other strains caused paralysis and death. Sequence analysis revealed several conserved genetic changes among these virus strains: nucleotide positions 107 and 648 in the 5′-untranslated region (UTR); amino acid position 88 in VP3; 1, 148, 282 and 283 in VP1; 22 in 2A; 47 in 3A. A series of chimeric and point-mutated infectious clones were constructed to identify viral elements responsible for the distinct virulence. A single amino acid change from isoleucine to valine at position 88 in VP3 attenuated neurovirulence by reducing virus replication in the brain and spinal cord of infected mice. View Full-Text
Keywords: enterovirus; enterovirus-D68; virulence determinant; mouse model; infectious clones; VP3; paralysis enterovirus; enterovirus-D68; virulence determinant; mouse model; infectious clones; VP3; paralysis
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MDPI and ACS Style

Yeh, M.T.; Capponi, S.; Catching, A.; Bianco, S.; Andino, R. Mapping Attenuation Determinants in Enterovirus-D68. Viruses 2020, 12, 867. https://doi.org/10.3390/v12080867

AMA Style

Yeh MT, Capponi S, Catching A, Bianco S, Andino R. Mapping Attenuation Determinants in Enterovirus-D68. Viruses. 2020; 12(8):867. https://doi.org/10.3390/v12080867

Chicago/Turabian Style

Yeh, Ming T.; Capponi, Sara; Catching, Adam; Bianco, Simone; Andino, Raul. 2020. "Mapping Attenuation Determinants in Enterovirus-D68" Viruses 12, no. 8: 867. https://doi.org/10.3390/v12080867

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