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Article

Isolation and Characterization of Bacteriophages That Infect Citrobacter rodentium, a Model Pathogen for Intestinal Diseases

1
Department of Microbiology, Institut Pasteur, 75015 Paris, France
2
Department of Biology, San Diego State University, San Diego, CA 92182, USA
3
Viral Information Institute, San Diego State University, San Diego, CA 92182, USA
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(7), 737; https://doi.org/10.3390/v12070737
Received: 2 May 2020 / Revised: 30 June 2020 / Accepted: 7 July 2020 / Published: 8 July 2020
(This article belongs to the Special Issue Phage Ecology)
Enteropathogenic Escherichia coli (EPEC) is a major pathogen for diarrheal diseases among children. Antibiotics, when used appropriately, are effective; however, their overuse and misuse have led to the rise of antibiotic resistance worldwide. Thus, there are renewed efforts into the development of phage therapy as an alternative antibacterial therapy. Because EPEC in vivo models have shortcomings, a surrogate is used to study the mouse pathogen Citrobacter rodentium in animal models. In this study, two new phages CrRp3 and CrRp10, which infect C. rodentium, were isolated and characterized. CrRp3 was found to be a new species within the genus Vectrevirus, and CrRp10 is a new strain within the species Escherichia virus Ime09, in the genus Tequatrovirus. Both phages appear to have independently evolved from E. coli phages, rather than other Citrobacter spp. phages. Neither phage strain carries known genes associated with bacterial virulence, antibiotic resistance, or lysogeny. CrRp3 is more potent, having a 24-fold faster adsorption rate and shorter lytic cycle when compared to the same properties of CrRp10. However, a lysis curve analysis revealed that CrRp10 prevented growth of C. rodentium for 18 h, whereas resistance developed against CrRp3 within 9 h. We also show that hypoxic (5% oxygen) conditions decreased CrRp3 ability to control bacterial densities in culture. In contrast, low oxygen conditions did not affect CrRp10 ability to replicate on C. rodentium. Together, CrRp10 is likely to be the better candidate for future phage therapy investigations. View Full-Text
Keywords: phages; phage therapy; hypoxic; resistance; Vectrevirus; vB_CroP_CrRp3; vB_CroP_CrRp10 phages; phage therapy; hypoxic; resistance; Vectrevirus; vB_CroP_CrRp3; vB_CroP_CrRp10
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MDPI and ACS Style

Mizuno, C.M.; Luong, T.; Cederstrom, R.; Krupovic, M.; Debarbieux, L.; Roach, D.R. Isolation and Characterization of Bacteriophages That Infect Citrobacter rodentium, a Model Pathogen for Intestinal Diseases. Viruses 2020, 12, 737. https://doi.org/10.3390/v12070737

AMA Style

Mizuno CM, Luong T, Cederstrom R, Krupovic M, Debarbieux L, Roach DR. Isolation and Characterization of Bacteriophages That Infect Citrobacter rodentium, a Model Pathogen for Intestinal Diseases. Viruses. 2020; 12(7):737. https://doi.org/10.3390/v12070737

Chicago/Turabian Style

Mizuno, Carolina M., Tiffany Luong, Robert Cederstrom, Mart Krupovic, Laurent Debarbieux, and Dwayne R. Roach. 2020. "Isolation and Characterization of Bacteriophages That Infect Citrobacter rodentium, a Model Pathogen for Intestinal Diseases" Viruses 12, no. 7: 737. https://doi.org/10.3390/v12070737

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