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Open AccessArticle

Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions

1
School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan
2
Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan
3
Division of Infection Control and Disease Prevention, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-0033, Japan
4
Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan
5
Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan
6
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-0033, Japan
*
Authors to whom correspondence should be addressed.
Viruses 2020, 12(7), 736; https://doi.org/10.3390/v12070736
Received: 16 June 2020 / Revised: 1 July 2020 / Accepted: 6 July 2020 / Published: 8 July 2020
(This article belongs to the Special Issue Recent Progress in Tumor Virology Research)
Papillomaviruses (PVs) usually cause benign proliferative lesions in the stratified epithelium of various animal species. However, some high-risk types of PVs have been proven to lead to malignant transformations. In dogs, several canine papillomaviruses (CPVs) have been identified in malignant lesions and are suggested as one of the risk factors for the development of squamous cell carcinomas (SCCs). In the present study, the full genomes of two CPV9 strains from recurrent SCCs of Dog 1 and skin viral papilloma (viral plaque) of Dog 2 were sequenced. Alignment of the two CPV9 sequences with the genome of the reference CPV9 strain (accession no. JF800656.1) derived from a solitary pigmented plaque was performed. Compared with the reference strain, a 27 bp in-frame insertion in the E1 gene was identified in both CPV9 strains in this study. In comparison with the CPV9 strains derived from benign lesions, the CPV9 from the SCCs of Dog 1 exhibited a 328 bp deletion at the 3′ end of the E2 and spacer sequence, which encoded a truncated deduced E2 protein and a chimeric E8^E2 protein. However, there was no difference in the mRNA expression levels of viral oncoproteins of E6 and E7 between the two CPV9 cases, suggesting that the oncogenesis of CPV9 for malignant transformation might be different from that of human papillomaviruses. The roles of E2 and E8^E2 deleted CPV9 in the oncogenesis of benign and malignant lesions should be further investigated. View Full-Text
Keywords: canine papillomavirus type 9 (CPV9); E6; E7; oncoprotein; squamous cell carcinoma (SCC); E2 canine papillomavirus type 9 (CPV9); E6; E7; oncoprotein; squamous cell carcinoma (SCC); E2
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MDPI and ACS Style

Chang, C.-Y.; Yamashita-Kawanishi, N.; Tomizawa, S.; Liu, I-L.; Chen, W.-T.; Chang, Y.-C.; Huang, W.-H.; Tsai, P.-S.; Shirota, K.; Chambers, J.K; Uchida, K.; Haga, T.; Chang, H.-W. Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions. Viruses 2020, 12, 736. https://doi.org/10.3390/v12070736

AMA Style

Chang C-Y, Yamashita-Kawanishi N, Tomizawa S, Liu I-L, Chen W-T, Chang Y-C, Huang W-H, Tsai P-S, Shirota K, Chambers JK, Uchida K, Haga T, Chang H-W. Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions. Viruses. 2020; 12(7):736. https://doi.org/10.3390/v12070736

Chicago/Turabian Style

Chang, Chia-Yu; Yamashita-Kawanishi, Nanako; Tomizawa, Sonoka; Liu, I-Li; Chen, Wei-Tao; Chang, Yen-Chen; Huang, Wei-Hsiang; Tsai, Pei-Shiue; Shirota, Kinji; Chambers, James K; Uchida, Kazuyuki; Haga, Takeshi; Chang, Hui-Wen. 2020. "Whole Genomic Analysis and Comparison of Two Canine Papillomavirus Type 9 Strains in Malignant and Benign Skin Lesions" Viruses 12, no. 7: 736. https://doi.org/10.3390/v12070736

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