TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Viruses, Cell Lines, Mosquitoes, and Antibodies
2.3. Pull-Down Assay and Mass Spectrometry
2.4. Immunoprecipitation and Immunoblotting
2.5. Immunofluorescence Assay and Confocal Microscopy
2.6. Generation of Stable Cell Lines Suppressing CCT2
2.7. Treatment of HSF1A and LDH Cytotoxicity Assay
2.8. Gene Silencing in Mosquitoes
2.9. Quantitative Real-Time PCR
2.10. Statistical Analysis
2.11. Data Availability
3. Results
3.1. TRiC/CCT Complex Interacts with the Zika Virus NS1 Protein
3.2. CCT2 Interacts with the Central Region of Zika Virus NS1
3.3. Interaction of ZIKV NS1 with CCT2 Depends on ATP Concentration
3.4. Knockdown of CCT2 Reduces Zika Virus Replication in Mammalian Cells
3.5. Inhibition of TRiC/CCT Complex Function Using HSF1A Reduces Zika Virus Propagation
3.6. Suppression of CCT2 Reduces Zika Virus Burden in Mosquitoes
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Protein ID | Protein Name | Mass (Da) | Sequence Coverage (%) | Mascot Score |
---|---|---|---|---|
Band 1 | ||||
GRP78_HUMAN | 78 kDa glucose-regulated protein Organism Name (OS) = Homo sapiens Gene Name (GN) = HSPA5 Protein Existence (PE) = 1 Sequence Version (SV) = 2 | 72,288 | 6.4 | 225 |
ZG16B_HUMAN | Zymogen granule protein 16 homolog B OS = Homo sapiens GN = ZG16B PE = 1 SV = 3 | 22,725 | 26 | 181 |
RORB_HUMAN | Nuclear receptor ROR-beta OS = Homo sapiens GN = RORB PE = 1 SV = 3 | 53,186 | 6 | 32 |
WFKN2_HUMAN | WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 OS = Homo sapiens GN = WFIKKN2 PE = 1 SV = 1 | 63,898 | 5.2 | 31 |
COJA1_HUMAN | Collagen alpha-1(XIX) chain OS = Homo sapiens GN = COL19A1 PE = 1 SV = 3 | 115,149 | 6.5 | 29 |
ARHGC_HUMAN | Rho guanine nucleotide exchange factor 12 OS = Homo sapiens GN = ARHGEF12 PE = 1 SV = 1 | 173,125 | 3.5 | 29 |
ZN469_HUMAN | Zinc finger protein 469 OS = Homo sapiens GN = ZNF469 PE = 2 SV = 3 | 409,949 | 1.1 | 26 |
KNG1_HUMAN | Kininogen-1 OS = Homo sapiens GN = KNG1 PE = 1 SV = 2 | 71,912 | 5.7 | 26 |
EEA1_HUMAN | Early endosome antigen 1 OS = Homo sapiens GN = EEA1 PE = 1 SV = 2 | 162,367 | 3.9 | 26 |
ATR_HUMAN | Serine/threonine-protein kinase ATR OS = Homo sapiens GN = ATR PE = 1 SV = 3 | 301,172 | 1.6 | 26 |
CIP1_HUMAN | E3 ubiquitin-protein ligase CCNB1IP1 OS = Homo sapiens GN = CCNB1IP1 PE = 1 SV = 1 | 31,524 | 2.9 | 25 |
ARSJ_HUMAN | Arylsulfatase J OS = Homo sapiens GN = ARSJ PE = 2 SV = 1 | 67,193 | 1.5 | 23 |
CC180_HUMAN | Coiled-coil domain-containing protein 180 OS = Homo sapiens GN = CCDC180 PE = 2 SV = 2 | 190,979 | 0.9 | 22 |
KI2S1_HUMAN | Killer cell immunoglobulin-like receptor 2DS1 OS = Homo sapiens GN = KIR2DS1 PE = 2 SV = 1 | 33,624 | 5.6 | 20 |
Band 2 | ||||
TCPB_HUMAN | T-complex protein 1 subunit beta OS = Homo sapiens GN = CCT2 PE = 1 SV = 4 | 57,452 | 47.9 | 1097 |
TCPQ_HUMAN | T-complex protein 1 subunit theta OS = Homo sapiens GN = CCT8 PE = 1 SV = 4 | 59,583 | 35.8 | 665 |
TCPH_HUMAN | T-complex protein 1 subunit eta OS = Homo sapiens GN = CCT7 PE = 1 SV = 2 | 59,329 | 32.2 | 627 |
TCPA_HUMAN | T-complex protein 1 subunit alpha OS = Homo sapiens GN = TCP1 PE = 1 SV = 1 | 60,306 | 36.5 | 623 |
TCPZ_HUMAN | T-complex protein 1 subunit zeta OS = Homo sapiens GN = CCT6A PE = 1 SV = 3 | 57,988 | 36 | 530 |
TCPG_HUMAN | T-complex protein 1 subunit gamma OS = Homo sapiens GN = CCT3 PE = 1 SV = 4 | 60,495 | 33.2 | 476 |
TCPE_HUMAN | T-complex protein 1 subunit epsilon OS = Homo sapiens GN = CCT5 PE = 1 SV = 1 | 59,633 | 30.9 | 458 |
TCPD_HUMAN | T-complex protein 1 subunit delta OS = Homo sapiens GN = CCT4 PE = 1 SV = 4 | 57,888 | 18 | 360 |
VIME_HUMAN | Vimentin OS=Homo sapiens GN = VIM PE = 1 SV = 4 | 53,619 | 12.2 | 97 |
S10AE_HUMAN | Protein S100-A14 OS = Homo sapiens GN = S100A14 PE = 1 SV = 1 | 11,655 | 14.4 | 95 |
CH60_HUMAN | 60 kDa heat shock protein, mitochondrial OS = Homo sapiens GN = HSPD1 PE = 1 SV = 2 | 61,016 | 3.3 | 59 |
KPYM_HUMAN | Pyruvate kinase PKM OS = Homo sapiens GN = PKM PE = 1 SV = 4 | 57,900 | 5.1 | 45 |
PRDX2_HUMAN | Peroxiredoxin-2 OS = Homo sapiens GN = PRDX2 PE = 1 SV = 5 | 21,878 | 10.1 | 43 |
ACTA_HUMAN | Actin, aortic smooth muscle OS = Homo sapiens GN = ACTA2 PE = 1 SV = 1 indistinguishable | 41,982 | 4.2 | 39 |
TBA1A_HUMAN | Tubulin alpha-1A chain OS = Homo sapiens GN = TUBA1A PE = 1 SV = 1 indistinguishable | 50,104 | 4 | 39 |
RHG29_HUMAN | Rho GTPase-activating protein 29 OS = Homo sapiens GN = ARHGAP29 PE = 1 SV = 2 | 141,974 | 3.3 | 37 |
ZFY27_HUMAN | Protrudin OS = Homo sapiens GN = ZFYVE27 PE = 1 SV = 1 | 45,814 | 3.4 | 35 |
RUVB1_HUMAN | RuvB-like 1 OS = Homo sapiens GN = RUVBL1 PE = 1 SV = 1 | 50,196 | 5 | 33 |
BLMH_HUMAN | Bleomycin hydrolase OS = Homo sapiens GN = BLMH PE = 1 SV = 1 | 52,528 | 2.4 | 33 |
TIAM2_HUMAN | T-lymphoma invasion and metastasis-inducing protein 2 OS = Homo sapiens GN = TIAM2 PE = 2 SV = 4 | 189,985 | 0.9 | 27 |
GON4L_HUMAN | GON-4-like protein OS = Homo sapiens GN = GON4L PE = 1 SV = 1 | 248,465 | 2.3 | 26 |
OR6S1_HUMAN | Olfactory receptor 6S1 OS = Homo sapiens GN = OR6S1 PE = 3 SV = 2 | 36,103 | 2.4 | 25 |
PKRI1_HUMAN | PRKR-interacting protein 1 OS = Homo sapiens GN = PRKRIP1 PE = 1 SV = 1 | 20,984 | 6.5 | 25 |
FBXL7_HUMAN | F-box/LRR-repeat protein 7 OS = Homo sapiens GN = FBXL7 PE = 2 SV = 1 | 54,540 | 3.1 | 25 |
RINI_HUMAN | Ribonuclease inhibitor OS = Homo sapiens GN = RNH1 PE = 1 SV = 2 | 49,941 | 2.6 | 24 |
KI26B_HUMAN | Kinesin-like protein KIF26B OS = Homo sapiens GN = KIF26B PE = 2 SV = 1 | 223,744 | 1.8 | 23 |
HMGB3_HUMAN | High mobility group protein B3 OS = Homo sapiens GN = HMGB3 PE = 1 SV = 4 | 22,965 | 4 | 23 |
RNF32_HUMAN | RING finger protein 32 OS = Homo sapiens GN = RNF32 PE = 1 SV = 1 | 41,490 | 1.9 | 22 |
BARD1_HUMAN | BRCA1-associated RING domain protein 1 OS = Homo sapiens GN = BARD1 PE = 1 SV = 2 | 86,593 | 3.3 | 21 |
TC1D4_HUMAN | Tctex1 domain-containing protein 4 OS = Homo sapiens GN = TCTEX1D4 PE = 1 SV = 1 | 23,338 | 3.2 | 21 |
PRA10_HUMAN | PRAME family member 10 OS = Homo sapiens GN = PRAMEF10 PE = 2 SV = 4 | 55,175 | 4 | 21 |
GTR7_HUMAN | Solute carrier family 2, facilitated glucose transporter member 7 OS = Homo sapiens GN = SLC2A7 PE = 2 SV = 2 | 55,692 | 1.6 | 20 |
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Wang, Y.; Uraki, R.; Hwang, J.; Fikrig, E. TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes. Viruses 2020, 12, 519. https://doi.org/10.3390/v12050519
Wang Y, Uraki R, Hwang J, Fikrig E. TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes. Viruses. 2020; 12(5):519. https://doi.org/10.3390/v12050519
Chicago/Turabian StyleWang, Yuchen, Ryuta Uraki, Jesse Hwang, and Erol Fikrig. 2020. "TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes" Viruses 12, no. 5: 519. https://doi.org/10.3390/v12050519