RNA Binding Suppresses Tsg101 Recognition of Ub-Modified Gag and Facilitates Recruitment to the Plasma Membrane
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production of Isolated Tsg101 UEV Domain
2.2. NMR Spectroscopy (Binding Studies)
2.3. Plasmids and Reagents
2.4. Transfection and Assays for Particle Budding
2.5. Fluorescence Microscopy
2.6. Yeast Two-Hybrid Assay
3. Results
3.1. The Tsg101 UEV Domain Binds tRNA
3.2. tRNA Binding Does Not Prevent Access to the Site Necessary for Mono-Ub Binding
3.3. The UEV Domain Is Not Required when Tsg101 Is at the Budding Site
3.4. Recruitment from the Cell Interior Requires the tRNA Binding Determinants
3.5. Mutation of tRNA Binding Determinants Promotes Recognition of Ub-Modified Gag
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Watanabe, S.M.; Strickland, M.; Tjandra, N.; Carter, C.A. RNA Binding Suppresses Tsg101 Recognition of Ub-Modified Gag and Facilitates Recruitment to the Plasma Membrane. Viruses 2020, 12, 447. https://doi.org/10.3390/v12040447
Watanabe SM, Strickland M, Tjandra N, Carter CA. RNA Binding Suppresses Tsg101 Recognition of Ub-Modified Gag and Facilitates Recruitment to the Plasma Membrane. Viruses. 2020; 12(4):447. https://doi.org/10.3390/v12040447
Chicago/Turabian StyleWatanabe, Susan M., Madeleine Strickland, Nico Tjandra, and Carol A. Carter. 2020. "RNA Binding Suppresses Tsg101 Recognition of Ub-Modified Gag and Facilitates Recruitment to the Plasma Membrane" Viruses 12, no. 4: 447. https://doi.org/10.3390/v12040447
APA StyleWatanabe, S. M., Strickland, M., Tjandra, N., & Carter, C. A. (2020). RNA Binding Suppresses Tsg101 Recognition of Ub-Modified Gag and Facilitates Recruitment to the Plasma Membrane. Viruses, 12(4), 447. https://doi.org/10.3390/v12040447