Porcine Circovirus 2 Induction of ROS Is Responsible for Mitophagy in PK-15 Cells via Activation of Drp1 Phosphorylation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Virus
2.2. Chemicals and Antibodies
2.3. Virus Infection and Chemical Treatments
2.4. Protein Extraction and SDS-PAGE/Western Blotting
2.5. Detection of Mitophagy
2.6. Transmission Electron Microscopy
2.7. Immunofluorescence
2.8. Measurement of the Mitochondrial Mass, ROS, and Mitochondrial Membrane Potential
2.9. Measurement of Apoptosis
2.10. Statistical Analysis
3. Results
3.1. PCV2 Induced Mitophagy with the Accumulation of Mitophagosomes
3.2. PCV2 Enhanced Drp1 Ser616 Phosphorylation and Its Mitochondrial Translocation
3.3. PCV2-Induced ROS was Involved in Drp1 Phosphorylation
3.4. PCV2-Induced Drp1 Phosphorylation and Mitophagy Could be Reversed Using ROS Scavenging N-acetyl-L-cysteine
3.5. Suppression of the Mitochondrial Fission Protein Drp1 Inhibited PCV2-Induced Mitophagy and Mitochondrial Apoptosis
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Zhang, Y.; Sun, R.; Li, X.; Fang, W. Porcine Circovirus 2 Induction of ROS Is Responsible for Mitophagy in PK-15 Cells via Activation of Drp1 Phosphorylation. Viruses 2020, 12, 289. https://doi.org/10.3390/v12030289
Zhang Y, Sun R, Li X, Fang W. Porcine Circovirus 2 Induction of ROS Is Responsible for Mitophagy in PK-15 Cells via Activation of Drp1 Phosphorylation. Viruses. 2020; 12(3):289. https://doi.org/10.3390/v12030289
Chicago/Turabian StyleZhang, Yikai, Renjie Sun, Xiaoliang Li, and Weihuan Fang. 2020. "Porcine Circovirus 2 Induction of ROS Is Responsible for Mitophagy in PK-15 Cells via Activation of Drp1 Phosphorylation" Viruses 12, no. 3: 289. https://doi.org/10.3390/v12030289