IRE1α Promotes Zika Virus Infection via XBP1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cells and Viruses
2.3. Expression Analysis
2.4. Immunofluorescence Microscopy
2.5. Western Blot
2.6. Plaque Assay
2.7. Mouse Model of ZIKV Infection
2.8. Statistics
3. Results
3.1. Zika Virus Infection Stimulates IRE1α Activation and Induction of XBP1 Targets
3.2. IRE1α Inhibitors Prevent Zika-Induced Cell Death
3.3. IRE1α and XBP1 Promote Zika Virus Replication in Cultured Cells
3.4. IRE1α and XBP1 Promote Zika Virus Infection in Mice
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kolpikova, E.P.; Tronco, A.R.; Den Hartigh, A.B.; Jackson, K.J.; Iwawaki, T.; Fink, S.L. IRE1α Promotes Zika Virus Infection via XBP1. Viruses 2020, 12, 278. https://doi.org/10.3390/v12030278
Kolpikova EP, Tronco AR, Den Hartigh AB, Jackson KJ, Iwawaki T, Fink SL. IRE1α Promotes Zika Virus Infection via XBP1. Viruses. 2020; 12(3):278. https://doi.org/10.3390/v12030278
Chicago/Turabian StyleKolpikova, Elena P., Ana R. Tronco, Andreas B. Den Hartigh, Konner J. Jackson, Takao Iwawaki, and Susan L. Fink. 2020. "IRE1α Promotes Zika Virus Infection via XBP1" Viruses 12, no. 3: 278. https://doi.org/10.3390/v12030278