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Volume 12
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10.3390/v12030259
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Article

Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein

by
Meng Hao
1,2,
Guanying Zhang
2,
Shengnan Zhang
3,
Zhengshan Chen
4,
Xiangyang Chi
2,
Yunzhu Dong
2,
Pengfei Fan
2,
Yujiao Liu
2,
Yi Chen
2,
Xiaohong Song
2,
Shuling Liu
2,
Changming Yu
2,*,
Jianmin Li
2,* and
Xianzhu Xia
1,5,*
1
Comparative Medicine Center, Peking Union Medical College (PUMC) and Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China
2
Beijing Institute of Biotechnology, Beijing 100071, China
3
College of Wildlife and Protected Areas, Northeast Forestry University, Harbin 150040, China
4
Tianjin Institue of Envionmental and Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin 300050, China
5
Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130000, China
*
Authors to whom correspondence should be addressed.
Viruses 2020, 12(3), 259; https://doi.org/10.3390/v12030259
Submission received: 3 February 2020 / Revised: 23 February 2020 / Accepted: 25 February 2020 / Published: 27 February 2020
(This article belongs to the Section Viral Immunology, Vaccines, and Antivirals)
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Abstract

The Rift Valley fever virus (RVFV) is an arthropod-borne virus that can not only cause severe disease in domestic animals but also in humans. However, the licensed vaccines or available therapeutics for humans do not exist. Here, we report two Gn-specific neutralizing antibodies (NAbs), isolated from a rhesus monkey immunized with recombinant human adenoviruses type 4 expressing Rift Valley fever virus Gn and Gc protein (rHAdV4-GnGcopt). The two NAbs were both able to protect host cells from RVFV infection. The interactions between NAbs and Gn were then characterized to demonstrate that these two NAbs might preclude RVFV glycoprotein rearrangement, hindering the exposure of fusion loops in Gc to endosomal membranes after the virus invades the host cell. The target region for the two NAbs is located in the Gn domain III, implying that Gn is a desired target for developing vaccines and neutralizing antibodies against RVFV.
Keywords: Rift Valley fever virus (RVFV); neutralizing antibody (NAb); Gn protein; critical residues Rift Valley fever virus (RVFV); neutralizing antibody (NAb); Gn protein; critical residues

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MDPI and ACS Style

Hao, M.; Zhang, G.; Zhang, S.; Chen, Z.; Chi, X.; Dong, Y.; Fan, P.; Liu, Y.; Chen, Y.; Song, X.; et al. Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein. Viruses 2020, 12, 259. https://doi.org/10.3390/v12030259

AMA Style

Hao M, Zhang G, Zhang S, Chen Z, Chi X, Dong Y, Fan P, Liu Y, Chen Y, Song X, et al. Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein. Viruses. 2020; 12(3):259. https://doi.org/10.3390/v12030259

Chicago/Turabian Style

Hao, Meng, Guanying Zhang, Shengnan Zhang, Zhengshan Chen, Xiangyang Chi, Yunzhu Dong, Pengfei Fan, Yujiao Liu, Yi Chen, Xiaohong Song, and et al. 2020. "Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein" Viruses 12, no. 3: 259. https://doi.org/10.3390/v12030259

APA Style

Hao, M., Zhang, G., Zhang, S., Chen, Z., Chi, X., Dong, Y., Fan, P., Liu, Y., Chen, Y., Song, X., Liu, S., Yu, C., Li, J., & Xia, X. (2020). Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein. Viruses, 12(3), 259. https://doi.org/10.3390/v12030259

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