Autochthonous Transmission of West Nile Virus by a New Vector in Iran, Vector-Host Interaction Modeling and Virulence Gene Determinants
Abstract
:1. Introduction
2. Material and Methods
2.1. Study Area and Sampling
2.2. Morphological and Molecular Identification of Mosquitoes
2.3. Blood Source Identification
2.4. Panel PCR for Arbovirus Screening
2.5. Polyprotein Characterization
2.6. Full Genome-Based Phylogenetic Tree Construction
3. Results
3.1. Mosquito Population Dynamic
3.2. Host-Vector Interaction
3.3. WNV Prevalence in Mosquito Specimens
3.4. Characterization of Full-Length Genome and Amino Acid Motifs
3.5. E-Protein Molecular Architecture
3.6. Genetic Determinants of Virulence in Iranian WNV Strains
3.7. Evolutionary Tree of WNV Based on Complete Nucleotide Sequences
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mosquito Species | No. of Mosquitoes/Species and (%) of Total | No. of Blood-Fed Mosquitoes/Species and (%) of Total | No. of Blood-Fed Mosquitoes/Host and (%) of Total | |||||
---|---|---|---|---|---|---|---|---|
Homo Sapiens | Sus Scrofa | Bos Taurus | Canis Lupus | Ovis Aries | Anas Sparsa | |||
Cx. pipiens Co. | 2885 (57.4) | 8 (10.5) | 5 (23.8) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 3 (42.9) |
Cx. theileri | 1224 (24.3) | 31 (40.8) | 13 (61.9) | 13 (72.2) | 3 (18.8) | 2 (18.2) | 0 (0) | 0 (0) |
Cx. perexiguus | 163 (3.2) | 11 (14.5) | 3 (14.3) | 3 (16.7) | 0 (0) | 1 (9.1) | 0 (0) | 4 (57.1) |
An. sacharovi | 535 (10.6) | 4 (5.3) | 0 (0) | 1 (5.6) | 1 (6.3) | 2 (18.2) | 0 (0) | 0 (0) |
An. stephensi | 83 (1.7) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
An. superpictus | 75 (1.5) | 2 (2.6) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 2 (66.7) | 0 (0) |
An. dthali | 32 (0.6) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
An. maculipennis | 28 (0.6) | 20 (26.3) | 0 (0) | 1 (5.6) | 12 (75) | 6 (54.5) | 1 (33.3) | 0 (0) |
An. fluviatilis | 3 (0.1) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
Total | 5028 (100) | 76 (100) | 21 | 18 | 16 | 11 | 3 | 7 |
Gene | Strain/Subclade/Pathogenicity | Iran (MN238670)/2g | Iran (MN238669)/2g | Russia (FJ425721)/2g/high | SA (EF429200) /2f/high | SA (EF429199)/2f/less | CAR (DQ318020)/2f/high | MAD (HM147823)/2e/attenuated | |
---|---|---|---|---|---|---|---|---|---|
a.a Position | |||||||||
C | - | - | - | - | - | - | - | - | |
prM | 73 | P | - | - | S | S | S | S | |
91 | - | - | - | - | - | K | - | ||
105 | A | - | - | - | V | - | - | ||
221 | A | - | - | G | - | - | - | ||
E | 66 | S | - | - | N | - | - | - | |
70 | T | - | - | P | - | - | - | ||
71 | K | - | - | R | R | R | - | ||
154 | N | - | - | - | - | D | - | ||
159 | M | - | - | I | I | I | V | ||
250 | Q | - | - | - | - | R | - | ||
312 | A | - | - | - | V | - | - | ||
NS1 | 88 | V | - | - | - | I | I | - | |
98 | K | - | - | - | - | R | - | ||
290 | S | - | - | - | R | - | - | ||
338 | T | - | - | - | - | - | L | ||
401 | I | - | V | - | - | - | - | ||
442 | L | - | M | - | - | - | - | ||
NS2A | 126 | S | - | - | - | - | - | L | |
190 | K | - | - | R | - | - | - | ||
259 | S | - | - | T | - | - | - | ||
NS2B | - | - | - | - | - | - | - | - | |
NS3 | 160 | S | - | - | - | A | - | A | |
298 | R | - | - | - | G | - | - | ||
333 | I | - | - | - | V | - | - | ||
421 | S | - | - | - | - | - | N | ||
572 | T | - | - | M | - | - | - | ||
597 | A | - | - | V | - | - | - | ||
NS4A | 98 | E | - | - | - | - | D | - | |
100 | P | - | S | - | - | - | - | ||
NS4B | 20 | P | - | - | - | - | - | L | |
117 | A | - | - | V | - | - | - | ||
274 | H | Y | Y | - | - | - | - | ||
NS5 | 42 | H | - | - | - | Q | - | - | |
117 | K | - | - | - | R | - | - | ||
254 | F | - | - | - | - | - | Y | ||
278 | K | - | - | - | R | - | - | ||
281 | N | - | - | - | S | - | - | ||
370 | E | - | A | - | - | - | - | ||
403 | R | - | - | G | G | G | - | ||
528 | P | - | - | - | G | - | S | ||
816 | A | - | - | S | - | S | - | ||
837 | K | - | - | R | - | R | - |
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Shahhosseini, N.; Moosa-Kazemi, S.H.; Sedaghat, M.M.; Wong, G.; Chinikar, S.; Hajivand, Z.; Mokhayeri, H.; Nowotny, N.; Kayedi, M.H. Autochthonous Transmission of West Nile Virus by a New Vector in Iran, Vector-Host Interaction Modeling and Virulence Gene Determinants. Viruses 2020, 12, 1449. https://doi.org/10.3390/v12121449
Shahhosseini N, Moosa-Kazemi SH, Sedaghat MM, Wong G, Chinikar S, Hajivand Z, Mokhayeri H, Nowotny N, Kayedi MH. Autochthonous Transmission of West Nile Virus by a New Vector in Iran, Vector-Host Interaction Modeling and Virulence Gene Determinants. Viruses. 2020; 12(12):1449. https://doi.org/10.3390/v12121449
Chicago/Turabian StyleShahhosseini, Nariman, Seyed Hassan Moosa-Kazemi, Mohammad Mehdi Sedaghat, Gary Wong, Sadegh Chinikar, Zahra Hajivand, Hamid Mokhayeri, Norbert Nowotny, and Mohammad Hassan Kayedi. 2020. "Autochthonous Transmission of West Nile Virus by a New Vector in Iran, Vector-Host Interaction Modeling and Virulence Gene Determinants" Viruses 12, no. 12: 1449. https://doi.org/10.3390/v12121449