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Open AccessArticle

Development of Genotype-Specific Anti-Bovine Rotavirus A Immunoglobulin Yolk Based on a Current Molecular Epidemiological Analysis of Bovine Rotaviruses A Collected in Japan during 2017–2020

1
Immunology Research Institute in Gifu, EW Nutrition Japan K.K., Gifu 501-1101, Japan
2
Division of Pathology and Pathophysiology, Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062-0045, Japan
3
Ehime Prefectural Livestock Disease Diagnostic Center, Toon, Ehime 791-0212, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Albert Bosch
Viruses 2020, 12(12), 1386; https://doi.org/10.3390/v12121386
Received: 5 November 2020 / Revised: 28 November 2020 / Accepted: 29 November 2020 / Published: 3 December 2020
(This article belongs to the Special Issue Enteric Viruses in Animals)
Bovine rotavirus A (RVA), a major causative pathogen of diarrhea in dairy and Japanese beef calves, has led to severe economic losses in numerous countries. A dual genotyping system based on genomic segments encoding VP7 (G genotype) and VP4 (P genotype), comprising the outer layer of the virion, has been used to understand the epidemiological dynamics of RVAs at the national and global levels. This study aimed to investigate occurrence frequency of G and P genotypes for multiple bovine RVAs from calf diarrheic samples collected in Japan from 2017 to 2020. After we produced anti-bovine RVA immunoglobulin yolks (IgYs) from hens immunized with the two RVAs with different genotypes (G6P[5] and G10P[11]) selected on the basis of the current epidemiological survey, we investigated cross-reactivity against bovine RVAs with different G and P combinations owing to establish a useful strategy to protect calves from RVA infections using the two IgYs. Consequently, the two produced anti-bovine IgYs showed strong cross-reactivity against bovine RVAs with the same G and/or P genotypes in neutralization assay, respectively. Therefore, our data suggest the possibility of a passive immunization to protect calves from a bovine RVA infections epidemic in Japan via oral administration of the two IgYs into calves. The findings presented herein will provide important information that IgY is one of the effective tools to prevent infections of various pathogens. View Full-Text
Keywords: calf; diarrhea; bovine rotavirus A; VP4 and VP7; G and P genotypes; immunoglobulin yolk; passive immunization calf; diarrhea; bovine rotavirus A; VP4 and VP7; G and P genotypes; immunoglobulin yolk; passive immunization
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MDPI and ACS Style

Odagiri, K.; Yoshizawa, N.; Sakihara, H.; Umeda, K.; Rahman, S.; Nguyen, S.V.; Suzuki, T. Development of Genotype-Specific Anti-Bovine Rotavirus A Immunoglobulin Yolk Based on a Current Molecular Epidemiological Analysis of Bovine Rotaviruses A Collected in Japan during 2017–2020. Viruses 2020, 12, 1386.

AMA Style

Odagiri K, Yoshizawa N, Sakihara H, Umeda K, Rahman S, Nguyen SV, Suzuki T. Development of Genotype-Specific Anti-Bovine Rotavirus A Immunoglobulin Yolk Based on a Current Molecular Epidemiological Analysis of Bovine Rotaviruses A Collected in Japan during 2017–2020. Viruses. 2020; 12(12):1386.

Chicago/Turabian Style

Odagiri, Koki; Yoshizawa, Nobuki; Sakihara, Hisae; Umeda, Koji; Rahman, Shofiqur; Nguyen, Sa V.; Suzuki, Tohru. 2020. "Development of Genotype-Specific Anti-Bovine Rotavirus A Immunoglobulin Yolk Based on a Current Molecular Epidemiological Analysis of Bovine Rotaviruses A Collected in Japan during 2017–2020" Viruses 12, no. 12: 1386.

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