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Communication

Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization

1
Centre de Recherche du CHUM, Montréal, QC H2X 0A9, Canada
2
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
3
Fred Hutchinson Cancer Research Center, Vaccines and Infectious Diseases Division, Seattle, 98109 WA, USA
4
Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institute of Health, Bethesda, 20892 MD, USA
5
Department of Global Health, University of Washington, Seattle, 98195 WA, USA
6
Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada
7
Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(11), 1214; https://doi.org/10.3390/v12111214
Received: 8 October 2020 / Accepted: 22 October 2020 / Published: 26 October 2020
(This article belongs to the Section SARS-CoV-2 and COVID-19)
Convalescent plasma from SARS-CoV-2 infected individuals and monoclonal antibodies were shown to potently neutralize viral and pseudoviral particles carrying the S glycoprotein. However, a non-negligent proportion of plasma samples from infected individuals, as well as S-specific monoclonal antibodies, were reported to be non-neutralizing despite efficient interaction with the S glycoprotein in different biochemical assays using soluble recombinant forms of S or when expressed at the cell surface. How neutralization relates to the binding of S glycoprotein in the context of viral particles remains to be established. Here, we developed a pseudovirus capture assay (VCA) to measure the capacity of plasma samples or antibodies immobilized on ELISA plates to bind to membrane-bound S glycoproteins from SARS-CoV-2 expressed at the surface of lentiviral particles. By performing VCA, ELISA, and neutralization assays, we observed a strong correlation between these parameters. However, while we found that plasma samples unable to capture viral particles did not neutralize, capture did not guarantee neutralization, indicating that the capacity of antibodies to bind to the S glycoprotein at the surface of pseudoviral particles is required but not sufficient to mediate neutralization. Altogether, our results highlight the importance of better understanding the inactivation of S by plasma and neutralizing antibodies. View Full-Text
Keywords: COVID-19; SARS-COV-2; convalescent plasma; ELISA; neutralization; virus capture assay COVID-19; SARS-COV-2; convalescent plasma; ELISA; neutralization; virus capture assay
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MDPI and ACS Style

Ding, S.; Laumaea, A.; Benlarbi, M.; Beaudoin-Bussières, G.; Gasser, R.; Medjahed, H.; Pancera, M.; Stamatatos, L.; McGuire, A.T.; Bazin, R.; Finzi, A. Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization. Viruses 2020, 12, 1214. https://doi.org/10.3390/v12111214

AMA Style

Ding S, Laumaea A, Benlarbi M, Beaudoin-Bussières G, Gasser R, Medjahed H, Pancera M, Stamatatos L, McGuire AT, Bazin R, Finzi A. Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization. Viruses. 2020; 12(11):1214. https://doi.org/10.3390/v12111214

Chicago/Turabian Style

Ding, Shilei; Laumaea, Annemarie; Benlarbi, Mehdi; Beaudoin-Bussières, Guillaume; Gasser, Romain; Medjahed, Halima; Pancera, Marie; Stamatatos, Leonidas; McGuire, Andrew T.; Bazin, Renée; Finzi, Andrés. 2020. "Antibody Binding to SARS-CoV-2 S Glycoprotein Correlates with but Does Not Predict Neutralization" Viruses 12, no. 11: 1214. https://doi.org/10.3390/v12111214

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