Next Article in Journal
Keeping It Together: Structures, Functions, and Applications of Viral Decoration Proteins
Next Article in Special Issue
The Unconventional Viruses of Ichneumonid Parasitoid Wasps
Previous Article in Journal
Identification of a New Equid Herpesvirus 1 DNA Polymerase (ORF30) Genotype with the Isolation of a C2254/H752 Strain in French Horses Showing no Major Impact on the Strain Behaviour
Previous Article in Special Issue
Fundamental Difficulties Prevent the Reconstruction of the Deep Phylogeny of Viruses
Open AccessArticle

A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides

1
Facultad de Ciencias Exactas Físicas y Naturales (FCEFyN), Universidad Nacional de Córdoba (UNC), Córdoba 5000, Argentina
2
Instituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Vélez Sarsfield 1611, Córdoba X5016GCA, Argentina
3
Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas (FCQ), Universidad Nacional de Córdoba (UNC), Córdoba 5000, Argentina
4
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Haya de la Torre y Medina Allende—Ciudad Universitaria, Córdoba X5000HUA, Argentina
5
Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga, Consejo Superior de Investigaciones Científicas (IHSM—UMA—CSIC), Estación Experimental “La Mayora”, Avenida Dr. Wienberg s/n, 29750 Algarrobo-Costa, Málaga, Spain
6
Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE—CIAP—INTA), Camino 60 Cuadras Km 5.5, Córdoba X5020ICA, Argentina
7
Estación Experimental Pergamino, (EEA) INTA Pergamino, Universidad Nacional Noroeste (UNNOBA), Pergamino (Buenos Aires) B2700, Argentina
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(10), 1161; https://doi.org/10.3390/v12101161
Received: 18 August 2020 / Revised: 28 September 2020 / Accepted: 9 October 2020 / Published: 14 October 2020
(This article belongs to the Collection Unconventional Viruses)
Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring. View Full-Text
Keywords: maize; sorghum; Fusarium verticillioides; Fusarium andiyazi; mycovirus; mitovirus FvMV1; FaMV1-162 maize; sorghum; Fusarium verticillioides; Fusarium andiyazi; mycovirus; mitovirus FvMV1; FaMV1-162
Show Figures

Graphical abstract

  • Supplementary File 1:

    PDF-Document (PDF, 367 KB)

  • Externally hosted supplementary file 1
    Doi: 10.5281/zenodo.3990533
    Link: https://zenodo.org/record/3990533
    Description: Predicted secondary structures (2-D representation of this self-folding) of the terminal untranslated regions (UTR) of FvMV1 and FaMV1-162. Schemes (a) and (c) represent the 5`-UTR and 3`-UTR of FvMV1 with dG = -82.52 and dG = -29.55, respectively. Schemes (b) and (d) represent the 5`-UTR and 3`-UTR of FaMV1-162 with dG = -73.96 and dG = -13.93, respectively. The +ssRNA molecules were folded, and the free energy was calculated with the RNA Folding Form V 2.3 Energies (MFOLD) program. For these calculations, the following conditions were sectioned: 25ºC, 1M NaCl and 0M divalent ions. The rendering of the structures has been defined with natural angles and annotated using colored base characters, based on p-num information. Colors are ranged from red to black representing the probability as well-determined (1) to poorly determined (0), respectively
MDPI and ACS Style

Jacquat, A.G.; Theumer, M.G.; Cañizares, M.C.; Debat, H.J.; Iglesias, J.; García Pedrajas, M.D.; Dambolena, J.S. A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides. Viruses 2020, 12, 1161.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop