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Open AccessArticle

A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh

1
International Centre of Excellence for Aquatic Animal Health, Centre for Environment, Fisheries and Aquaculture Sciences (Cefas), Weymouth, Dorset DT4 8UB, UK
2
WorldFish Bangladesh, Dhaka 1213, Bangladesh
3
Winrock Bangladesh, Dhaka 1212, Bangladesh
4
Centre for Sustainable Aquaculture Futures, University of Exeter, Stocker Road, Exeter EX4 4QY, UK
5
WorldFish, Penang 11960, Malaysia
6
Bangladesh Fisheries Research Institute, Shrimp Research Station, Bagerhat 9300, Bangladesh
7
Department of Life Sciences, the Natural History Museum, London SW7 5BD, UK
*
Authors to whom correspondence should be addressed.
Viruses 2020, 12(10), 1120; https://doi.org/10.3390/v12101120
Received: 5 September 2020 / Revised: 28 September 2020 / Accepted: 29 September 2020 / Published: 2 October 2020
Mass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single-stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of Macrobrachium rosenbergii golda virus (MrGV) suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture. View Full-Text
Keywords: Macrobrachium rosenbergii; freshwater prawns; Nidovirales; Macrobrachium rosenbergii Golda virus; RNA virus; Roniviridae; emerging disease; aquaculture Macrobrachium rosenbergii; freshwater prawns; Nidovirales; Macrobrachium rosenbergii Golda virus; RNA virus; Roniviridae; emerging disease; aquaculture
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MDPI and ACS Style

Hooper, C.; Debnath, P.P.; Biswas, S.; van Aerle, R.; Bateman, K.S.; Basak, S.K.; Rahman, M.M.; Mohan, C.V.; Islam, H.M.R.; Ross, S.; Stentiford, G.D.; Currie, D.; Bass, D. A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh. Viruses 2020, 12, 1120. https://doi.org/10.3390/v12101120

AMA Style

Hooper C, Debnath PP, Biswas S, van Aerle R, Bateman KS, Basak SK, Rahman MM, Mohan CV, Islam HMR, Ross S, Stentiford GD, Currie D, Bass D. A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh. Viruses. 2020; 12(10):1120. https://doi.org/10.3390/v12101120

Chicago/Turabian Style

Hooper, Chantelle; Debnath, Partho P.; Biswas, Sukumar; van Aerle, Ronny; Bateman, Kelly S.; Basak, Siddhawartha K.; Rahman, Muhammad M.; Mohan, Chadag V.; Islam, H. M.R.; Ross, Stuart; Stentiford, Grant D.; Currie, David; Bass, David. 2020. "A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh" Viruses 12, no. 10: 1120. https://doi.org/10.3390/v12101120

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