Detection of Human Papillomaviruses in the Nasopharynx of Breastfed Infants: New Findings and Meta-Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects and Biological Samples
2.2. DNA Extraction
2.3. HPV Detection and Genotyping
2.4. Meta-Analysis
2.5. Statistical Analysis
3. Results
Meta-Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Patient Variability | Cases n | HPV Negative n (%) | HPV Positive n (%) | Alpha HPV n (%) | Beta HPV n (%) | |
---|---|---|---|---|---|---|
Age | <2 months (T1) | 38 | 36 (94.7%) | 2 (5.3%) | 0 (0%) | 2 (5.3%) |
2–6 months (T2) | 65 | 54 (83.1%) | 11 (16.9%) | 0 (0%) | 11 (16.9%) | |
>6 months (T3) | 10 | 9 (90%) | 1 (10%) | 0 (0%) | 1 (10%) | |
TOT | 113 | 99 (87.6%) | 14 (12.4%) | 0 (0%) | 14 (12.4%) | |
Sex | Male | 61 | 56 (91.8%) | 5 (8.2%) | 0 (0%) | 5 (8.2%) |
Female | 52 | 43 (82.7%) | 9 (17.3%) | 0 (0%) | 9 (17.3%) |
Beta HPV Genotype (Genus, Species) | Cases n = 14 (%) | T1 (<2 m) n = 38 (%) | T2 (2 m–6 m) n = 65 (%) | T3 (>6 m) n = 10 (%) |
---|---|---|---|---|
HPV 20 (β,1) | 3 (21.4%) | 0 (0%) | 3 (4.6%) | 0 (0%) |
HPV 107 (β,2) | 3 (21.4%) | 1 (2.6%) | 2 (3%) | 0 (0%) |
HPV 100 (β,2) | 2 (14.3%) | 0 (0%) | 2 (3%) | 0 (0%) |
RTRX7 (β,1) | 2 (14.3%) | 1 (2.6%) | 1 (1.5%) | 0 (0%) |
HPV 124 (β,1) | 1 (7.1%) | 0 (0%) | 0 (0%) | 1 (10%) |
HPV 25 (β,1) | 1 (7.1%) | 0 (0%) | 1 (1.5%) | 0 (0%) |
HPV 5b (β,1) | 1 (7.1%) | 0 (0%) | 1 (1.5%) | 0 (0%) |
HPV 152 (β,1) | 1 (7.1%) | 0 (0%) | 1 (1.5%) | 0 (0%) |
TOT | 14 (12.4%) | 2 (5.3%) | 11 (16.9%) | 1 (10%) |
Alpha HPV | Beta HPV | |||
---|---|---|---|---|
Age | HPV + /Total | % | HPV + /Total | % |
T0: at birth | 282/3298 | 8.5% | 2/18 | 11.1% |
T1: 1–30 days | 108/689 | 15.7% | 24/36 | 66.7% |
T2: >1–12 months | 90/708 | 12.7% | 18/35 | 51.4% |
T3: >12 months | 7/77 | 9.1% | 21/33 | 63.6% |
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Dassi, L.; Annunziata, C.; Botti, C.; Micillo, A.; Cerasuolo, A.; Starita, N.; Buonaguro, F.M.; Tornesello, M.L. Detection of Human Papillomaviruses in the Nasopharynx of Breastfed Infants: New Findings and Meta-Analysis. Viruses 2020, 12, 1119. https://doi.org/10.3390/v12101119
Dassi L, Annunziata C, Botti C, Micillo A, Cerasuolo A, Starita N, Buonaguro FM, Tornesello ML. Detection of Human Papillomaviruses in the Nasopharynx of Breastfed Infants: New Findings and Meta-Analysis. Viruses. 2020; 12(10):1119. https://doi.org/10.3390/v12101119
Chicago/Turabian StyleDassi, Luisa, Clorinda Annunziata, Chiara Botti, Alberto Micillo, Andrea Cerasuolo, Noemy Starita, Franco M. Buonaguro, and Maria Lina Tornesello. 2020. "Detection of Human Papillomaviruses in the Nasopharynx of Breastfed Infants: New Findings and Meta-Analysis" Viruses 12, no. 10: 1119. https://doi.org/10.3390/v12101119