HIF-1α Modulates Core Metabolism and Virus Replication in Primary Airway Epithelial Cells Infected with Respiratory Syncytial Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. RSV Preparation, Cell Culture and Virus Infection
2.2. Metabolomics Assay
2.3. Western Blots
2.4. Quantitative Real-Time PCR
2.5. Statistical Analysis
3. Results
3.1. RSV Infection Induces a Shift in Core Metabolic Pathways in Human Primary Airway Epithelial Cells
3.2. RSV Infection Stabilizes Expression of HIF-1α in SAE and Immortalized SAE Cells
3.3. Suppression of HIF-1α Decreases Key Enzymes of the Glycolytic Pathway
3.4. HIF-2α Expression Does Not Modulate Glycolytic Pathway in RSV Infection
3.5. Inhibition of HIF-1α Reduces Viral Replication
4. Discussions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Morris, D.R.; Qu, Y.; Agrawal, A.; Garofalo, R.P.; Casola, A. HIF-1α Modulates Core Metabolism and Virus Replication in Primary Airway Epithelial Cells Infected with Respiratory Syncytial Virus. Viruses 2020, 12, 1088. https://doi.org/10.3390/v12101088
Morris DR, Qu Y, Agrawal A, Garofalo RP, Casola A. HIF-1α Modulates Core Metabolism and Virus Replication in Primary Airway Epithelial Cells Infected with Respiratory Syncytial Virus. Viruses. 2020; 12(10):1088. https://doi.org/10.3390/v12101088
Chicago/Turabian StyleMorris, Dorothea R., Yue Qu, Anurodh Agrawal, Roberto P. Garofalo, and Antonella Casola. 2020. "HIF-1α Modulates Core Metabolism and Virus Replication in Primary Airway Epithelial Cells Infected with Respiratory Syncytial Virus" Viruses 12, no. 10: 1088. https://doi.org/10.3390/v12101088
APA StyleMorris, D. R., Qu, Y., Agrawal, A., Garofalo, R. P., & Casola, A. (2020). HIF-1α Modulates Core Metabolism and Virus Replication in Primary Airway Epithelial Cells Infected with Respiratory Syncytial Virus. Viruses, 12(10), 1088. https://doi.org/10.3390/v12101088