Single Amino Acid Substitutions in the Cucumber Mosaic Virus 1a Protein Induce Necrotic Cell Death in Virus-Inoculated Leaves without Affecting Virus Multiplication
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plants and Virus
2.2. In Vitro Transcription of Infectious CMV RNA and Production of Reassortant CMV
2.3. Virus Inoculation and Detection
2.4. Virus Quantification by ELISA
2.5. Detection of Cell Death
2.6. RNA-Seq Analysis
2.7. Construction of In Vitro Transcription Vectors Carrying Chimeric cDNA of CMV RNA1
2.8. Single Amino Acid Substitution in 1a Proteins Encoded on RNA1 of CMV(Y)
3. Results
3.1. Response of Arabidopsis thaliana Ecotype Col-0 to a Series of Reassortant CMVs
3.2. Response of A. thaliana Ecotypes to CMV(HYY)
3.3. Comparison of Global Gene Expression Pattern between Two Types of Cell Death in Arabidopsis Leaves
3.4. Analysis of the Viral Sequence in CMV RNA1 Inducing Necrotic Cell Death in Virus-Inoculated Leaves
3.5. Analysis of Single Amino Acid Substitutions in the CMV 1a Protein for Induction of Necrotic Cell Death in Virus-Inoculated Leaves
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Ethical Approval
References
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Tian, A.; Miyashita, S.; Ando, S.; Takahashi, H. Single Amino Acid Substitutions in the Cucumber Mosaic Virus 1a Protein Induce Necrotic Cell Death in Virus-Inoculated Leaves without Affecting Virus Multiplication. Viruses 2020, 12, 91. https://doi.org/10.3390/v12010091
Tian A, Miyashita S, Ando S, Takahashi H. Single Amino Acid Substitutions in the Cucumber Mosaic Virus 1a Protein Induce Necrotic Cell Death in Virus-Inoculated Leaves without Affecting Virus Multiplication. Viruses. 2020; 12(1):91. https://doi.org/10.3390/v12010091
Chicago/Turabian StyleTian, Ainan, Shuhei Miyashita, Sugihiro Ando, and Hideki Takahashi. 2020. "Single Amino Acid Substitutions in the Cucumber Mosaic Virus 1a Protein Induce Necrotic Cell Death in Virus-Inoculated Leaves without Affecting Virus Multiplication" Viruses 12, no. 1: 91. https://doi.org/10.3390/v12010091