Interaction of a Densovirus with Glycans of the Peritrophic Matrix Mediates Oral Infection of the Lepidopteran Pest Spodoptera frugiperda
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insect Rearing and Virus Preparation
2.2. Calcofluor, Glycans and Lectins
2.3. Insect Bioassays
2.4. Hemagglutination Assay
2.5. PM Isolation, Ex Vivo Infection and Microscopic Observation
2.6. Midgut Semi-Thin Sections and Immunolabelling
2.7. Chitin Binding Assay and Pull Down
2.8. SDS-PAGE, PAS Staining and VOPBA
2.9. Proteomic LC-MS/MS Analysis and Data Processing
2.10. RNA Extraction, DGE Library Construction and Sequencing
2.11. Transcriptomic Analysis of the Midgut
3. Results
3.1. The Peritrophic Matrix of S. frugiperda is a Barrier to JcDV Infection
3.2. JcDV Binding to the PM is Required for Oral Infection of S. frugiperda Caterpillars
3.3. JcDV Binds to Both Chitin and Protein Components of the PM
3.4. JcDV Early Pathogenesis Induces Changes in Midgut Metabolism
4. Discussion
4.1. JcDV Recognition and Binding to Glycans
4.2. The Role of PM Glycans in the Species Barrier to Densovirus Infection
4.3. JcDV Early Infection Induces Physiological Changes in the Midgut
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Pigeyre, L.; Schatz, M.; Ravallec, M.; Gasmi, L.; Nègre, N.; Clouet, C.; Seveno, M.; El Koulali, K.; Decourcelle, M.; Guerardel, Y.; et al. Interaction of a Densovirus with Glycans of the Peritrophic Matrix Mediates Oral Infection of the Lepidopteran Pest Spodoptera frugiperda. Viruses 2019, 11, 870. https://doi.org/10.3390/v11090870
Pigeyre L, Schatz M, Ravallec M, Gasmi L, Nègre N, Clouet C, Seveno M, El Koulali K, Decourcelle M, Guerardel Y, et al. Interaction of a Densovirus with Glycans of the Peritrophic Matrix Mediates Oral Infection of the Lepidopteran Pest Spodoptera frugiperda. Viruses. 2019; 11(9):870. https://doi.org/10.3390/v11090870
Chicago/Turabian StylePigeyre, Laetitia, Malvina Schatz, Marc Ravallec, Leila Gasmi, Nicolas Nègre, Cécile Clouet, Martial Seveno, Khadija El Koulali, Mathilde Decourcelle, Yann Guerardel, and et al. 2019. "Interaction of a Densovirus with Glycans of the Peritrophic Matrix Mediates Oral Infection of the Lepidopteran Pest Spodoptera frugiperda" Viruses 11, no. 9: 870. https://doi.org/10.3390/v11090870