Aichi Virus Induces Antiviral Host Defense in Primary Murine Intestinal Epithelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus, Cell Lines, and Reagents
2.2. Isolation of Mouse Intestinal Epithelial Cells
2.3. Aichi Virus Infection and Plaque Assay
2.4. Immunofluorescence Assay
2.5. Quantitative Reverse Transcription-Polymerase Chain Reaction(qRT-PCR)
2.6. Western Blot Analysis
2.7. Cell Viability and Cytotoxicity Assay
2.8. Statistical Analysis
3. Results
3.1. Isolation of Primary Mouse Intestinal Epithelial Cells for Toll-Like receptor-Ligand Stimulation
3.2. Productive Infection of Aichi Virus in Intestinal Epithelial Cells
3.3. Aichi Virus-Induced Type I Interferons and Expression of Inflammatory Cytokines in Intestinal Epithelial Cells
3.4. Aichi Virus Activated Antiviral Response in T84 Human Intestinal Epithelial Cells
4. Discussion
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Chang, Y.-T.; Kung, M.-H.; Hsu, T.-H.; Hung, W.-T.; Chen, Y.-S.; Yen, L.-C.; Chang, T.-H. Aichi Virus Induces Antiviral Host Defense in Primary Murine Intestinal Epithelial Cells. Viruses 2019, 11, 763. https://doi.org/10.3390/v11080763
Chang Y-T, Kung M-H, Hsu T-H, Hung W-T, Chen Y-S, Yen L-C, Chang T-H. Aichi Virus Induces Antiviral Host Defense in Primary Murine Intestinal Epithelial Cells. Viruses. 2019; 11(8):763. https://doi.org/10.3390/v11080763
Chicago/Turabian StyleChang, Yun-Te, Ming-Hsiang Kung, Thung-Hsien Hsu, Wan-Ting Hung, Yao-Shen Chen, Li-Chen Yen, and Tsung-Hsien Chang. 2019. "Aichi Virus Induces Antiviral Host Defense in Primary Murine Intestinal Epithelial Cells" Viruses 11, no. 8: 763. https://doi.org/10.3390/v11080763