Next Article in Journal
Genetic Variability of Chikungunya Virus in Southern Mexico
Next Article in Special Issue
Approach to Strain Selection and the Propagation of Viral Stocks for Venezuelan Equine Encephalitis Virus Vaccine Efficacy Testing under the Animal Rule
Previous Article in Journal
Aerosol Detection and Transmission of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): What Is the Evidence, and What Are the Knowledge Gaps?
Open AccessCommunication

Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion

Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life & Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia
Royal North Shore Hospital, Clinical Microbiology & Infectious Diseases, Reserve Road, St Leonards, NSW 2065, Australia
Vets & Veterinary Surgeons, Jerry Plains Veterinary Hospital, 10 Pagan Street, Jerry Plains, NSW 2330, Australia
Author to whom correspondence should be addressed.
Viruses 2019, 11(8), 713;
Received: 9 July 2019 / Revised: 30 July 2019 / Accepted: 1 August 2019 / Published: 4 August 2019
(This article belongs to the Special Issue Equine Viruses)
Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequencing (meta-transcriptomics) we identified a novel equine papillomavirus in semen taken from a thoroughbred stallion suffering a genital lesion, which was confirmed by nested RT-PCR. We designate this novel virus Equus caballus papillomavirus 9 (EcPV9). The complete 7656 bp genome of EcPV9 exhibited similar characteristics to those of other horse papillomaviruses. Phylogenetic analysis based on concatenated E1-E2-L2-L1 amino acid sequences revealed that EcPV9 clustered with EcPV2, EcPV4, and EcPV5, although was distinct enough to represent a new viral species within the genus Dyoiotapapillomavirus (69.35%, 59.25%, and 58.00% nucleotide similarity to EcPV2, EcPV4, and EcPV5, respectively). In sum, we demonstrate the presence of a novel equine papillomavirus for which more detailed studies of disease association are merited. View Full-Text
Keywords: equine papillomaviruses; horse; genital wart; phylogeny; evolution equine papillomaviruses; horse; genital wart; phylogeny; evolution
Show Figures

Figure 1

MDPI and ACS Style

Li, C.-X.; Chang, W.-S.; Mitsakos, K.; Rodger, J.; Holmes, E.C.; Hudson, B.J. Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion. Viruses 2019, 11, 713.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop