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Open AccessArticle

The Kaposi’s Sarcoma-Associated Herpesvirus Protein ORF42 Is Required for Efficient Virion Production and Expression of Viral Proteins

1
Graduate Program in Biochemistry, Sackler School of Graduate Biomedical Sciences, Tufts University, 136 Harrison Ave, Boston, MA 02111, USA
2
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111, USA
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(8), 711; https://doi.org/10.3390/v11080711
Received: 14 July 2019 / Revised: 29 July 2019 / Accepted: 30 July 2019 / Published: 2 August 2019
(This article belongs to the Section Animal Viruses)
Kaposi’s sarcoma-associated herpesvirus (KSHV), the etiologic agent of Kaposi’s sarcoma and other aggressive AIDS-associated malignancies, encodes over 90 genes, most of which are expressed only during the lytic replication cycle. The role of many of the KSHV lytic proteins in the KSHV replication cycle remains unknown, and many proteins are annotated based on known functions of homologs in other herpesviruses. Here we investigate the role of the previously uncharacterized KSHV lytic protein ORF42, a presumed tegument protein. We find that ORF42 is dispensable for reactivation from latency but is required for efficient production of viral particles. Like its alpha- and beta-herpesviral homologs, ORF42 is a late protein that accumulates in the viral particles. However, unlike its homologs, ORF42 appears to be required for efficient expression of at least some viral proteins and may potentiate post-transcriptional stages of gene expression. These results demonstrate that ORF42 has an important role in KSHV replication and may contribute to shaping viral gene expression. View Full-Text
Keywords: KSHV; ORF42; late gene expression; herpesvirus; herpes UL7 family KSHV; ORF42; late gene expression; herpesvirus; herpes UL7 family
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MDPI and ACS Style

Butnaru, M.; Gaglia, M.M. The Kaposi’s Sarcoma-Associated Herpesvirus Protein ORF42 Is Required for Efficient Virion Production and Expression of Viral Proteins. Viruses 2019, 11, 711.

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