Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus
Abstract
1. Introduction
2. Materials and Methods
2.1. Clinical Samples
2.2. Construction of Synthetic Standard PUUV RNA
2.3. Primers and Probes
2.4. PUUV-qRT-PCR
2.5. RT-Nested-PCR
2.6. Detection of Immunoglobulins
3. Results
3.1. PUUV-qRT-PCR Is Both Sensitive and Specific
3.2. RT-Nested-PCR and Subsequent Sequencing Shows the High Variability of PUUV Strains Detected
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Patient No | Sex | Age | Hospital District | IgM | PUUV-qRT-PCR | RT-Nested-PCR |
---|---|---|---|---|---|---|
1 | F | 39 | HUS | pos | pos | pos |
2 | M | 25 | HUS | pos | pos | NEG |
3 | F | 38 | HUS | pos | pos | pos |
4 | M | 55 | HUS | pos | NEG | NEG |
5 | F | 45 | HUS | pos | NEG | NEG |
6 | M | 65 | HUS | pos | pos | pos |
7 | F | 30 | HUS | pos | pos | NEG |
8 | F | 61 | LP | pos | pos | pos |
9 | F | 26 | HUS | pos | pos | pos |
10 | M | 57 | HUS | pos | pos | pos |
11 | F | 47 | HUS | pos | pos | pos |
12 | F | 64 | HUS | pos | pos | pos |
13 | M | 29 | KYM | pos | pos | pos |
14 | F | 61 | HUS | pos | pos | pos |
15 | F | 32 | HUS | pos | pos | pos |
16 | M | 46 | HUS | pos | pos | pos |
17 | F | 65 | KYM | pos | pos | pos |
18 | M | 66 | HUS | pos | pos | NEG |
19 | M | 71 | SO | pos | pos | pos |
20 | M | 77 | TP | pos | pos | pos |
21 | M | 48 | CF | pos | pos | pos |
22 | F | 28 | HUS | pos | pos | pos |
23 | M | 50 | HUS | pos | pos | pos |
24 | M | 39 | HUS | pos | pos | pos |
25 | M | 51 | CF | pos | pos | pos |
26 | F | 66 | HUS | pos | pos | pos |
27 | F | 51 | HUS | pos | pos | pos |
28 | M | 43 | TP | pos | pos | pos |
29 | M | 54 | HUS | pos | pos | pos |
30 | M | 57 | HUS | pos | pos | pos |
No of positives: | 30 | 28 | 25 |
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Niskanen, S.; Jääskeläinen, A.; Vapalahti, O.; Sironen, T. Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus. Viruses 2019, 11, 661. https://doi.org/10.3390/v11070661
Niskanen S, Jääskeläinen A, Vapalahti O, Sironen T. Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus. Viruses. 2019; 11(7):661. https://doi.org/10.3390/v11070661
Chicago/Turabian StyleNiskanen, Silja, Anne Jääskeläinen, Olli Vapalahti, and Tarja Sironen. 2019. "Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus" Viruses 11, no. 7: 661. https://doi.org/10.3390/v11070661
APA StyleNiskanen, S., Jääskeläinen, A., Vapalahti, O., & Sironen, T. (2019). Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus. Viruses, 11(7), 661. https://doi.org/10.3390/v11070661