Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity

Bai, Meng; Cheng, Ya-Hui; Sun, Xue-Qin; Wang, Zi-Yi; Wang, Yong-Xia; Cui, Xiao-Long; Xiao, Wei

doi:10.3390/v11070615

Open AccessArticle

Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity

by

Meng Bai

¹,

Ya-Hui Cheng

²,

Xue-Qin Sun

¹,

Zi-Yi Wang

²,

Yong-Xia Wang

¹,

Xiao-Long Cui

^1,3,* and

Wei Xiao

^1,4,*

¹

Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091, China

²

Yunnan Engineering Laboratory of Soil Fertility and Pollution Remediation, Yunnan Agricultural University, Kunming 650201, China

³

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, China

⁴

Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming 650500, China

^*

Authors to whom correspondence should be addressed.

Viruses 2019, 11(7), 615; https://doi.org/10.3390/v11070615

Submission received: 28 April 2019 / Revised: 18 June 2019 / Accepted: 2 July 2019 / Published: 5 July 2019

(This article belongs to the Special Issue Diversity and Evolution of Phage Genomes)

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Abstract

Aeromonas species are common pathogens of fish and some of them can opportunistically cause infectious diseases in humans. The overuse of antibiotics has led to the emergence of bacterial drug-resistance. To date, only 51 complete genome sequences of Aeromonas phages are available in GenBank. Here, we report the isolation of nine Aeromonas phages from a plateau lake in China. The protein cluster, dot plot and ANI analyses were performed on all 60 currently sequenced Aeromonas phage genomes and classified into nine clusters and thirteen singletons. Among the nine isolated phages, the DNA-packaging strategy of cluster 2L372D (including 2L372D, 2L372X, 4L372D, 4L372XY) is unknown, while the other five phages use the headful (P22/Sf6) DNA-packaging strategy. Notably, the isolated phages with larger genomes conservatively encode auxiliary metabolism genes, DNA replication and metabolism genes, while in smaller phage genomes, recombination-related genes were conserved. Finally, we propose a new classification scheme for Aeromonas phages.

Keywords: bacteriophage; Aeromonas; genome evolution; large terminase; auxiliary metabolism gene

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MDPI and ACS Style

Bai, M.; Cheng, Y.-H.; Sun, X.-Q.; Wang, Z.-Y.; Wang, Y.-X.; Cui, X.-L.; Xiao, W. Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity. Viruses 2019, 11, 615. https://doi.org/10.3390/v11070615

AMA Style

Bai M, Cheng Y-H, Sun X-Q, Wang Z-Y, Wang Y-X, Cui X-L, Xiao W. Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity. Viruses. 2019; 11(7):615. https://doi.org/10.3390/v11070615

Chicago/Turabian Style

Bai, Meng, Ya-Hui Cheng, Xue-Qin Sun, Zi-Yi Wang, Yong-Xia Wang, Xiao-Long Cui, and Wei Xiao. 2019. "Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity" Viruses 11, no. 7: 615. https://doi.org/10.3390/v11070615

APA Style

Bai, M., Cheng, Y.-H., Sun, X.-Q., Wang, Z.-Y., Wang, Y.-X., Cui, X.-L., & Xiao, W. (2019). Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity. Viruses, 11(7), 615. https://doi.org/10.3390/v11070615

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity

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