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Bacillus Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New Siphoviridae Genus

1
Laboratory of Bacteriophage Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, 142290 Pushchino, Russia
2
Department of Microbiology, Federal State Budgetary Educational Institution of Higher Education “Vyatka State University”, 610000 Kirov, Russia
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(7), 624; https://doi.org/10.3390/v11070624
Received: 2 June 2019 / Revised: 28 June 2019 / Accepted: 5 July 2019 / Published: 7 July 2019
(This article belongs to the Special Issue Diversity and Evolution of Phage Genomes)
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Abstract

The Bacillus cereus group of bacteria includes, inter alia, the species known to be associated with human diseases and food poisoning. Here, we describe the Bacillus phage vB_BtS_B83 (abbreviated as B83) infecting the species of this group. Transmission electron microscopy (TEM) micrographs indicate that B83 belongs to the Siphoviridae family. B83 is a temperate phage using an arbitrium system for the regulation of the lysis–lysogeny switch, and is probably capable of forming a circular plasmid prophage. Comparative analysis shows that it has been previously sequenced, but was mistaken for a plasmid. B83 shares common genome organization and >46% of proteins with other the Bacillus phage, BMBtp14. Phylograms constructed using large terminase subunits and a pan-genome presence–absence matrix show that these phages form a clade distinct from the closest viruses. Based on the above, we propose the creation of a new genus named Bembunaquatrovirus that includes B83 and BMBtp14. View Full-Text
Keywords: bacteriophage; phage; Bacillus thuringiensis; plasmid prophage; Bembunaquatrovirus; Siphoviridae bacteriophage; phage; Bacillus thuringiensis; plasmid prophage; Bembunaquatrovirus; Siphoviridae
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Piligrimova, E.G.; Kazantseva, O.A.; Nikulin, N.A.; Shadrin, A.M. Bacillus Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New Siphoviridae Genus. Viruses 2019, 11, 624.

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