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Genetic Variability of Chrysodeixis Includens Nucleopolyhedrovirus (ChinNPV) and the Insecticidal Characteristics of Selected Genotypic Variants

1
Institute for Multidisciplinary Research in Applied Biology, Universidad Pública de Navarra, 31006 Pamplona, Navarra, Spain
2
Departamento de Investigación y Desarrollo, Bioinsectis SL, Pol. Ind. Mocholi Plaza Cein 5, Nave A14, 31110 Noain, Navarra, Spain
3
Instituto de Ecología AC, Xalapa 91073, Veracruz, Mexico
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(7), 581; https://doi.org/10.3390/v11070581
Received: 24 May 2019 / Revised: 14 June 2019 / Accepted: 21 June 2019 / Published: 26 June 2019
(This article belongs to the Special Issue Insect Viruses and Pest Management)
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Abstract

Genetic variation in baculoviruses is recognized as a key factor, not only due to the influence of such variation on pathogen transmission and virulence traits, but also because genetic variants can form the basis for novel biological insecticides. In this study, we examined the genetic variability of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) present in field isolates obtained from virus-killed larvae. Different ChinNPV strains were identified by restriction endonuclease analysis, from which genetic variants were isolated by plaque assay. Biological characterization studies were based on pathogenicity, median time to death (MTD), and viral occlusion body (OB) production (OBs/larva). Nine different isolates were obtained from eleven virus-killed larvae collected from fields of soybean in Mexico. An equimolar mixture of these isolates, named ChinNPV-Mex1, showed good insecticidal properties and yielded 23 genetic variants by plaque assay, one of which (ChinNPV-R) caused the highest mortality in second instars of C. includens. Five of these variants were selected: ChinNPV-F, ChinNPV-J, ChinNPV-K, ChinNPV-R, and ChinNPV-V. No differences in median time to death were found between them, while ChinNPV-F, ChinNPV-K, ChinNPV-R and ChinNPV-V were more productive than ChinNPV-J and the original mixture of field isolates ChinNPV-Mex1. These results demonstrate the high variability present in natural populations of this virus and support the use of these new genetic variants as promising active substances for baculovirus-based bioinsecticides. View Full-Text
Keywords: genetic variants; plaque assay; bioassay; pathogenicity; speed-of-kill; bioinsecticide genetic variants; plaque assay; bioassay; pathogenicity; speed-of-kill; bioinsecticide
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Aguirre, E.; Beperet, I.; Williams, T.; Caballero, P. Genetic Variability of Chrysodeixis Includens Nucleopolyhedrovirus (ChinNPV) and the Insecticidal Characteristics of Selected Genotypic Variants. Viruses 2019, 11, 581.

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