Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy
AbstractThe aim of this study was to gain further insight into the diversity of Escherichia coli phages
followed by enhanced work on taxonomic issues in that field. Therefore, we present the genomic
characterization and taxonomic classification of 50 bacteriophages against E. coli isolated from
various sources, such as manure or sewage. All phages were examined for their host range on a set
of different E. coli strains, originating, e.g., from human diagnostic laboratories or poultry farms.
Transmission electron microscopy revealed a diversity of morphotypes (70% Myo-, 22% Sipho-, and
8% Podoviruses), and genome sequencing resulted in genomes sizes from ~44 to ~370 kb.
Annotation and comparison with databases showed similarities in particular to T4- and T5-like
phages, but also to less-known groups. Though various phages against E. coli are already described
in literature and databases, we still isolated phages that showed no or only few similarities to other
phages, namely phages Goslar, PTXU04, and KWBSE43-6. Genome-based phylogeny and
classification of the newly isolated phages using VICTOR resulted in the proposal of new genera
and led to an enhanced taxonomic classification of E. coli phages.
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Korf, I.H.E.; Meier-Kolthoff, J.P.; Adriaenssens, E.M.; Kropinski, A.M.; Nimtz, M.; Rohde, M.; van Raaij, M.J.; Wittmann, J. Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy. Viruses 2019, 11, 454.
Korf IHE, Meier-Kolthoff JP, Adriaenssens EM, Kropinski AM, Nimtz M, Rohde M, van Raaij MJ, Wittmann J. Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy. Viruses. 2019; 11(5):454.Chicago/Turabian Style
Korf, Imke H. E.; Meier-Kolthoff, Jan P.; Adriaenssens, Evelien M.; Kropinski, Andrew M.; Nimtz, Manfred; Rohde, Manfred; van Raaij, Mark J.; Wittmann, Johannes. 2019. "Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy." Viruses 11, no. 5: 454.
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