Next Article in Journal
A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization
Previous Article in Journal
Development of a Versatile, Near Full Genome Amplification and Sequencing Approach for a Broad Variety of HIV-1 Group M Variants
Article Menu

Export Article

Open AccessArticle
Viruses 2019, 11(4), 318; https://doi.org/10.3390/v11040318

Characterization of Two Pseudomonas aeruginosa Viruses vB_PaeM_SCUT-S1 and vB_PaeM_SCUT-S2

School of Biology and Biological Engineering, South China University of Technology, 382 East Outer Loop Road, Higher Education Mega Centre, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
Received: 5 January 2019 / Revised: 17 March 2019 / Accepted: 28 March 2019 / Published: 1 April 2019
(This article belongs to the Section Bacterial Viruses)
  |  
PDF [4192 KB, uploaded 16 April 2019]
  |  

Abstract

The sophisticated antibiotic resistance mechanism of Pseudomonas aeruginosa has urged the development of alternative antibacterial strategies. Phage therapy has been proven successful for the treatment of multidrug-resistant infections. In this study, we reported two virulent P. aeruginosa phages, vB_PaeM_SCUT-S1 (S1) and vB_PaeM_SCUT-S2 (S2), which were characterized at morphological, genomic, and proteomic levels. Phages S1 and S2 were assigned to the Myoviridae family. The genome sequencing showed that the genome size of Phage S1 was 66,046 bp and that of Phage S2 was 94,434 bp. The phylogenetic tree indicated that the two phages were distantly related to each other and were classified in the genera Pbunavirus and Pakpunavirus respectively. Thirty-one proteins were identified for each phage by mass spectrometry and were used to substantiate the function of the predicted coding genes. The two phages inhibited the growth of P. aeruginosa strain PAO1 at low multiplicity of infection levels and had good performance both on preventing biofilm formation and eradicating preformed biofilms. They were also stable over a wide range of temperature and pH values, supporting their potential use in the treatment of P. aeruginosa infections. View Full-Text
Keywords: Pseudomonas aeruginosa; phage therapy; Myoviridae; complete genome; proteomics; one-step growth curve; lysis kinetics; biofilm Pseudomonas aeruginosa; phage therapy; Myoviridae; complete genome; proteomics; one-step growth curve; lysis kinetics; biofilm
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Guo, Y.; Chen, P.; Lin, Z.; Wang, T. Characterization of Two Pseudomonas aeruginosa Viruses vB_PaeM_SCUT-S1 and vB_PaeM_SCUT-S2. Viruses 2019, 11, 318.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top