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Self-Assembly of Tail Tube Protein of Bacteriophage vB_EcoS_NBD2 into Extremely Long Polytubes in E. coli and S. cerevisiae

1
Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio al. 7, LT-10257 Vilnius, Lithuania
2
Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio al. 7, LT-10257 Vilnius, Lithuania
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(3), 208; https://doi.org/10.3390/v11030208
Received: 23 January 2019 / Revised: 22 February 2019 / Accepted: 26 February 2019 / Published: 1 March 2019
(This article belongs to the Section Bacterial Viruses)
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Abstract

Nucleotides, peptides and proteins serve as a scaffold material for self-assembling nanostructures. In this study, the production of siphovirus vB_EcoS_NBD2 (NBD2) recombinant tail tube protein gp39 reached approximately 33% and 27% of the total cell protein level in Escherichia coli and Saccharomyces cerevisiae expression systems, respectively. A simple purification protocol allowed us to produce a recombinant gp39 protein with 85%–90% purity. The yield of gp39 was 2.9 ± 0.36 mg/g of wet E. coli cells and 0.85 ± 0.33 mg/g for S. cerevisiae cells. The recombinant gp39 self-assembled into well-ordered tubular structures (polytubes) in vivo in the absence of other phage proteins. The diameter of these structures was the same as the diameter of the tail of phage NBD2 (~12 nm). The length of these structures varied from 0.1 µm to >3.95 µm, which is 23-fold the normal NBD2 tail length. Stability analysis demonstrated that the polytubes could withstand various chemical and physical conditions. These polytubes show the potential to be used as a nanomaterial in various fields of science. View Full-Text
Keywords: bacteriophage vB_EcoS_NBD2; tail tube protein; self-assembly; tubular structure; polytubes; stability; Saccharomyces cerevisiae; Escherichia coli bacteriophage vB_EcoS_NBD2; tail tube protein; self-assembly; tubular structure; polytubes; stability; Saccharomyces cerevisiae; Escherichia coli
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Špakova, A.; Šimoliūnas, E.; Batiuškaitė, R.; Pajeda, S.; Meškys, R.; Petraitytė-Burneikienė, R. Self-Assembly of Tail Tube Protein of Bacteriophage vB_EcoS_NBD2 into Extremely Long Polytubes in E. coli and S. cerevisiae. Viruses 2019, 11, 208.

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