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Discovery and Characterization of Bukakata orbivirus (Reoviridae:Orbivirus), a Novel Virus from a Ugandan Bat

Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
Department of Zoology, Entomology and Fisheries Sciences, Makerere University, Kampala, Uganda
Arboviral Diseases Branch, Division of Vector-borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80523, USA
Department of Arbovirology, Emerging, and Re-emerging Viral Infections, Uganda Virus Research Institute, Entebbe, Uganda
Department of Biosecurity, Ecosystems and Veterinary Public Health, Makerere University, Kampala, Uganda
National Wildlife Research Center, US Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, Fort Collins, CO 80521, USA
College of Arts and Sciences, Roosevelt University, Collections & Research, The Field Museum of Natural History, Chicago, IL 60605, USA
Viral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
United States Public Health Service, Commissioned Corps, Rockville, MD 20852, USA
Author to whom correspondence should be addressed.
Viruses 2019, 11(3), 209;
Received: 14 February 2019 / Revised: 26 February 2019 / Accepted: 26 February 2019 / Published: 2 March 2019
(This article belongs to the Special Issue Viruses and Bats 2019)
While serological and virological evidence documents the exposure of bats to medically-important arboviruses, their role as reservoirs or amplifying hosts is less well-characterized. We describe a novel orbivirus (Reoviridae:Orbivirus) isolated from an Egyptian fruit bat (Rousettus aegyptiacus leachii) trapped in 2013 in Uganda and named Bukakata orbivirus. This is the fifth orbivirus isolated from a bat, however genetic information had previously only been available for one bat-associated orbivirus. We performed whole-genome sequencing on Bukakata orbivirus and three other bat-associated orbiviruses (Fomede, Ife, and Japanaut) to assess their phylogenetic relationship within the genus Orbivirus and develop hypotheses regarding potential arthropod vectors. Replication kinetics were assessed for Bukakata orbivirus in three different vertebrate cell lines. Lastly, qRT-PCR and nested PCR were used to determine the prevalence of Bukakata orbivirus RNA in archived samples from three populations of Egyptian fruit bats and one population of cave-associated soft ticks in Uganda. Complete coding sequences were obtained for all ten segments of Fomede, Ife, and Japanaut orbiviruses and for nine of the ten segments for Bukakata orbivirus. Phylogenetic analysis placed Bukakata and Fomede in the tick-borne orbivirus clade and Ife and Japanaut within the Culicoides/phlebotomine sandfly orbivirus clade. Further, Bukakata and Fomede appear to be serotypes of the Chobar Gorge virus species. Bukakata orbivirus replicated to high titers (106–107 PFU/mL) in Vero, BHK-21 [C-13], and R06E (Egyptian fruit bat) cells. Preliminary screening of archived bat and tick samples do not support Bukakata orbivirus presence in these collections, however additional testing is warranted given the phylogenetic associations observed. This study provided complete coding sequence for several bat-associated orbiviruses and in vitro characterization of a bat-associated orbivirus. Our results indicate that bats may play an important role in the epidemiology of viruses in the genus Orbivirus and further investigation is warranted into vector-host associations and ongoing surveillance efforts. View Full-Text
Keywords: arbovirus; bat; orbivirus; next-generation sequencing; surveillance; Reoviridae arbovirus; bat; orbivirus; next-generation sequencing; surveillance; Reoviridae
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MDPI and ACS Style

Fagre, A.C.; Lee, J.S.; Kityo, R.M.; Bergren, N.A.; Mossel, E.C.; Nakayiki, T.; Nalikka, B.; Nyakarahuka, L.; Gilbert, A.T.; Peterhans, J.K.; Crabtree, M.B.; Towner, J.S.; Amman, B.R.; Sealy, T.K.; Schuh, A.J.; Nichol, S.T.; Lutwama, J.J.; Miller, B.R.; Kading, R.C. Discovery and Characterization of Bukakata orbivirus (Reoviridae:Orbivirus), a Novel Virus from a Ugandan Bat. Viruses 2019, 11, 209.

AMA Style

Fagre AC, Lee JS, Kityo RM, Bergren NA, Mossel EC, Nakayiki T, Nalikka B, Nyakarahuka L, Gilbert AT, Peterhans JK, Crabtree MB, Towner JS, Amman BR, Sealy TK, Schuh AJ, Nichol ST, Lutwama JJ, Miller BR, Kading RC. Discovery and Characterization of Bukakata orbivirus (Reoviridae:Orbivirus), a Novel Virus from a Ugandan Bat. Viruses. 2019; 11(3):209.

Chicago/Turabian Style

Fagre, Anna C., Justin S. Lee, Robert M. Kityo, Nicholas A. Bergren, Eric C. Mossel, Teddy Nakayiki, Betty Nalikka, Luke Nyakarahuka, Amy T. Gilbert, Julian K. Peterhans, Mary B. Crabtree, Jonathan S. Towner, Brian R. Amman, Tara K. Sealy, Amy J. Schuh, Stuart T. Nichol, Julius J. Lutwama, Barry R. Miller, and Rebekah C. Kading. 2019. "Discovery and Characterization of Bukakata orbivirus (Reoviridae:Orbivirus), a Novel Virus from a Ugandan Bat" Viruses 11, no. 3: 209.

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