Next Article in Journal
Viruses of Polar Aquatic Environments
Next Article in Special Issue
Classifying the Unclassified: A Phage Classification Method
Previous Article in Journal
Tomato Chlorotic Spot Virus (TCSV) Putatively Incorporated a Genomic Segment of Groundnut Ringspot Virus (GRSV) Upon a Reassortment Event
Previous Article in Special Issue
Phage Reduce Stability for Regaining Infectivity during Antagonistic Coevolution with Host Bacterium
Open AccessArticle

Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System

Department of Biological Sciences, Universidad de Los Andes, 111711 Bogotá, Colombia
Instituto René Rachou, Fundação Oswaldo Cruz, 21040-900 Belo Horizonte, Brazil
Instituto Tecnológico Vale, 66055-090 Belém, Brazil
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
Author to whom correspondence should be addressed.
Viruses 2019, 11(2), 188;
Received: 22 December 2018 / Revised: 4 February 2019 / Accepted: 13 February 2019 / Published: 22 February 2019
(This article belongs to the Special Issue Diversity and Evolution of Phage Genomes)
Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy. View Full-Text
Keywords: phage-therapy; Salmonella Enteritidis; bacteria-phage coevolution; antibiotics; bacterial resistance phage-therapy; Salmonella Enteritidis; bacteria-phage coevolution; antibiotics; bacterial resistance
Show Figures

Figure 1

MDPI and ACS Style

Holguín, A.V.; Cárdenas, P.; Prada-Peñaranda, C.; Rabelo Leite, L.; Buitrago, C.; Clavijo, V.; Oliveira, G.; Leekitcharoenphon, P.; Møller Aarestrup, F.; Vives, M.J. Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. Viruses 2019, 11, 188.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop