The C4 Protein from Tomato Yellow Leaf Curl Virus Can Broadly Interact with Plant Receptor-Like Kinases
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Plasmids and Cloning
2.3. Transient Expression in N. benthamiana
2.4. Protein Extraction and Co-Immunoprecipitation
2.5. FRET-FLIM
2.6. Hormone and Peptide Treatments
2.7. RNA Extraction and qRT-PCR
2.8. Detection of Reactive Oxygen Species (ROS)
2.9. Phylogeny and Protein Sequence Alignment
3. Results and Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Garnelo Gómez, B.; Zhang, D.; Rosas-Díaz, T.; Wei, Y.; Macho, A.P.; Lozano-Durán, R. The C4 Protein from Tomato Yellow Leaf Curl Virus Can Broadly Interact with Plant Receptor-Like Kinases. Viruses 2019, 11, 1009. https://doi.org/10.3390/v11111009
Garnelo Gómez B, Zhang D, Rosas-Díaz T, Wei Y, Macho AP, Lozano-Durán R. The C4 Protein from Tomato Yellow Leaf Curl Virus Can Broadly Interact with Plant Receptor-Like Kinases. Viruses. 2019; 11(11):1009. https://doi.org/10.3390/v11111009
Chicago/Turabian StyleGarnelo Gómez, Borja, Dan Zhang, Tábata Rosas-Díaz, Yali Wei, Alberto P. Macho, and Rosa Lozano-Durán. 2019. "The C4 Protein from Tomato Yellow Leaf Curl Virus Can Broadly Interact with Plant Receptor-Like Kinases" Viruses 11, no. 11: 1009. https://doi.org/10.3390/v11111009