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Open AccessArticle

Analysis of Swine Conventional Dendritic Cells, DEC205+CD172a+/−CADM1+, from Blood and Spleen in Response to PRRSV and PEDV

Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Kilómetro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico
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Viruses 2019, 11(11), 1001; https://doi.org/10.3390/v11111001
Received: 26 September 2019 / Revised: 28 October 2019 / Accepted: 30 October 2019 / Published: 31 October 2019
(This article belongs to the Special Issue Porcine Viruses 2019)
Conventional dendritic cells (cDCs) cannot be infected by porcine reproductive and respiratory syndrome virus (PRRSV) but respond to infection via cytokine production, indicating a possible role in initiation/regulation of the immune response against PRRSV. In this work, we evaluated the responses of splenic and blood cDCs, with DEC205+CADM1+CD172a+/− phenotype, as well as those of CD163+ cells against PRRSV and porcine epidemic diarrhea virus (PEDV). Both populations were incubated in the presence of PRRSV or PEDV with and without naïve CD3+ T cells, and cytokine responses were evaluated by qPCR and ELISA. Our results showed that cDCs, but not CD163+ cells, produced IL-12 in response to PRRSV. PEDV did not induce IL-12 production. Cocultures of cDCs and autologous naïve CD3+ cells resulted in decreased IL-12 production and low expression of IFN-γ transcripts in response to PRRSV. Interestingly, cDCs increased the proliferation of naïve T cells in the presence of PRRSV compared with that achieved with monocytes and peripheral blood mononuclear cells (PBMCs). Cocultures of CD163+ cells induced IL-10 and IL-4 expression in the presence of PRRSV and PEDV, respectively. In conclusion, cDCs can selectively produce IL-12 in response to PRRSV but poorly participate in the activation of naïve T cells. View Full-Text
Keywords: dendritic cells; cDC; DEC205; PRRSV; PEDV; IL-12; naïve T cells dendritic cells; cDC; DEC205; PRRSV; PEDV; IL-12; naïve T cells
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Parra-Sánchez, H.; Bustamante-Córdova, L.; Reséndiz, M.; Mata-Haro, V.; Pinelli-Saavedra, A.; Hernández, J. Analysis of Swine Conventional Dendritic Cells, DEC205+CD172a+/−CADM1+, from Blood and Spleen in Response to PRRSV and PEDV. Viruses 2019, 11, 1001.

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