Next Article in Journal
ATM and ATR Expression Potentiates HBV Replication and Contributes to Reactivation of HBV Infection upon DNA Damage
Previous Article in Journal
Analysis of Swine Conventional Dendritic Cells, DEC205+CD172a+/−CADM1+, from Blood and Spleen in Response to PRRSV and PEDV
Open AccessArticle

Capsular Polysaccharide Is a Receptor of a Clostridium perfringens Bacteriophage CPS1

Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul 03722, Korea
Authors to whom correspondence should be addressed.
Viruses 2019, 11(11), 1002;
Received: 6 September 2019 / Revised: 26 October 2019 / Accepted: 29 October 2019 / Published: 31 October 2019
(This article belongs to the Section Bacterial Viruses)
Clostridium perfringens is a Gram-positive, anaerobic, and spore forming bacterium that is widely distributed in the environment and one of the most common causes of foodborne illnesses. Bacteriophages are regarded as one of the most promising alternatives to antibiotics in controlling antibiotic-resistant pathogenic bacteria. Here we isolated a virulent C. perfringens phage, CPS1, and analysis of its whole genome and morphology revealed a small genome (19 kbps) and a short noncontractile tail, suggesting that CPS1 can be classified as a member of Picovirinae, a subfamily of Podoviridae. To determine the host receptor of CPS1, the EZ-Tn5 random transposon mutant library of C. perfringens ATCC 13124 was constructed and screened for resistance to CPS1 infection. Analysis of the CPS1-resistant mutants revealed that the CPF_0486 was disrupted by Tn5. The CPF_0486 was annotated as galE, a gene encoding UDP-glucose 4-epimerase (GalE). However, biochemical analyses demonstrated that the encoded protein possessed dual activities of GalE and UDP-N-acetylglucosamine 4-epimerase (Gne). We found that the CPF_0486::Tn5 mutant produced a reduced amount of capsular polysaccharides (CPS) compared with the wild type. We also discovered that glucosamine and galactosamine could competitively inhibit host adsorption of CPS1. These results suggest that CPS acts as a receptor for this phage. View Full-Text
Keywords: bacteriophage; Clostridium perfringens; phage receptor; capsular polysaccharides bacteriophage; Clostridium perfringens; phage receptor; capsular polysaccharides
Show Figures

Figure 1

MDPI and ACS Style

Ha, E.; Chun, J.; Kim, M.; Ryu, S. Capsular Polysaccharide Is a Receptor of a Clostridium perfringens Bacteriophage CPS1. Viruses 2019, 11, 1002.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop