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Open AccessArticle

Capsular Polysaccharide Is a Receptor of a Clostridium perfringens Bacteriophage CPS1

1
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
2
Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul 03722, Korea
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(11), 1002; https://doi.org/10.3390/v11111002
Received: 6 September 2019 / Revised: 26 October 2019 / Accepted: 29 October 2019 / Published: 31 October 2019
(This article belongs to the Section Bacterial Viruses)
Clostridium perfringens is a Gram-positive, anaerobic, and spore forming bacterium that is widely distributed in the environment and one of the most common causes of foodborne illnesses. Bacteriophages are regarded as one of the most promising alternatives to antibiotics in controlling antibiotic-resistant pathogenic bacteria. Here we isolated a virulent C. perfringens phage, CPS1, and analysis of its whole genome and morphology revealed a small genome (19 kbps) and a short noncontractile tail, suggesting that CPS1 can be classified as a member of Picovirinae, a subfamily of Podoviridae. To determine the host receptor of CPS1, the EZ-Tn5 random transposon mutant library of C. perfringens ATCC 13124 was constructed and screened for resistance to CPS1 infection. Analysis of the CPS1-resistant mutants revealed that the CPF_0486 was disrupted by Tn5. The CPF_0486 was annotated as galE, a gene encoding UDP-glucose 4-epimerase (GalE). However, biochemical analyses demonstrated that the encoded protein possessed dual activities of GalE and UDP-N-acetylglucosamine 4-epimerase (Gne). We found that the CPF_0486::Tn5 mutant produced a reduced amount of capsular polysaccharides (CPS) compared with the wild type. We also discovered that glucosamine and galactosamine could competitively inhibit host adsorption of CPS1. These results suggest that CPS acts as a receptor for this phage. View Full-Text
Keywords: bacteriophage; Clostridium perfringens; phage receptor; capsular polysaccharides bacteriophage; Clostridium perfringens; phage receptor; capsular polysaccharides
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Ha, E.; Chun, J.; Kim, M.; Ryu, S. Capsular Polysaccharide Is a Receptor of a Clostridium perfringens Bacteriophage CPS1. Viruses 2019, 11, 1002.

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