Epidemiology of Deltacoronaviruses (δ-CoV) and Gammacoronaviruses (γ-CoV) in Wild Birds in the United States
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples and RNA Extraction
2.2. γ- and δ-CoV RT-PCR Assays
2.3. Sequencing and Sequence Analysis
3. Results
3.1. Detection of γ-CoV in Wild Birds
3.2. Detection of δ-CoV in Wild Birds
3.3. Sequence and Phylogenetic Analyses of δ-CoVs
3.4. HKU20-Specific RT-PCR and Partial N Gene Sequence and Phylogenetic Analyses of δ-CoVs
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Bird Species | γ-CoV+ | δ-CoV+ | Total |
---|---|---|---|
American green-winged teal | 27/24.5% | 0/0% | 110 |
American wigeon | 1/5.6% | 0/0% | 18 |
Blue-winged teal | 27/21.4% | 6/4.8% | 126 |
Mallard | 16/7% | 2/0.9% | 227 |
Northern pintail | 1/8.3% | 0/0% | 12 |
Northern shoveler | 0/0% | 1/16.7% | 6 |
Red-tailed hawk | 0/0% | 1/100% | 1 |
Ring-necked duck | 1/4.3% | 0/0% | 23 |
Snow goose | 0/0% | 4/5.8% | 69 |
Deltacoronavirus | Sample Collection Date | RT-PCR Results | Sequencing Results | ||
---|---|---|---|---|---|
UDCoV | HKU20 | Next Generation | Partial N-Gene | ||
Snow goose coronavirus/USA/Arkansas0009/2015 | 1/29/2015 | + | N/A* | N/A | N/A |
Snow goose coronavirus/USA/Arkansas0012/2015 | 1/30/2015 | + | N/A | N/A | N/A |
Snow goose coronavirus/USA/Arkansas0014/2015 | 1/30/2015 | + | N/A | N/A | N/A |
Snow goose coronavirus/USA/Arkansas0017/2015 | 1/30/2015 | + | N/A | N/A | N/A |
Red-tailed hawk coronavirus/USA/Ohio1248/2015 | 5/3/2015 | + | N/A | N/A | N/A |
Mallard coronavirus/USA/Ohio4381/2015 | 8/4/2015 | + | - | Did not yield CoV sequences | N/A |
Norther shoveler coronavirus/USA/Mississippi8042/2015 | 12/22/2015 | + | N/A | N/A | N/A |
Blue-winged teal coronavirus/USA/Illinois2662/2017 | 12/22/2015 | + | + | N/A | Most closely related to HKU20 |
Blue-winged teal coronavirus/USA/Missouri3057/2017 | 10/16/2017 | + | + | N/A | N/A |
Blue-winged teal coronavirus/USA/Missouri3230/2017 | 10/23/2017 | + | + | Did not yield CoV sequences | Most closely related to HKU20 |
Environmental coronavirus/USA/Maryland3464/2017 | 10/23/2017 | + | - | N/A | N/A |
Blue-winged teal coronavirus/USA/Illinois2537/2017 | 10/16/2017 | + | + | N/A | N/A |
Blue-winged teal coronavirus/USA/Illinois2562/2017 | 10/16/2017 | + | + | Yielded 85 δ-CoV sequences from ORF1a/b, S, M, N/NS7a: most closely related to HKU20 | Most closely related to HKU20 |
Blue-winged teal coronavirus/USA/Illinois2615/2017 | 10/16/2017 | + | + | Did not yield CoV sequences | Most closely related to HKU20 |
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Paim, F.C.; Bowman, A.S.; Miller, L.; Feehan, B.J.; Marthaler, D.; Saif, L.J.; Vlasova, A.N. Epidemiology of Deltacoronaviruses (δ-CoV) and Gammacoronaviruses (γ-CoV) in Wild Birds in the United States. Viruses 2019, 11, 897. https://doi.org/10.3390/v11100897
Paim FC, Bowman AS, Miller L, Feehan BJ, Marthaler D, Saif LJ, Vlasova AN. Epidemiology of Deltacoronaviruses (δ-CoV) and Gammacoronaviruses (γ-CoV) in Wild Birds in the United States. Viruses. 2019; 11(10):897. https://doi.org/10.3390/v11100897
Chicago/Turabian StylePaim, Francine C., Andrew S. Bowman, Lauren Miller, Brandi J. Feehan, Douglas Marthaler, Linda J. Saif, and Anastasia N. Vlasova. 2019. "Epidemiology of Deltacoronaviruses (δ-CoV) and Gammacoronaviruses (γ-CoV) in Wild Birds in the United States" Viruses 11, no. 10: 897. https://doi.org/10.3390/v11100897
APA StylePaim, F. C., Bowman, A. S., Miller, L., Feehan, B. J., Marthaler, D., Saif, L. J., & Vlasova, A. N. (2019). Epidemiology of Deltacoronaviruses (δ-CoV) and Gammacoronaviruses (γ-CoV) in Wild Birds in the United States. Viruses, 11(10), 897. https://doi.org/10.3390/v11100897