Next Article in Journal
Plant-Derived Purification, Chemical Synthesis, and In Vitro/In Vivo Evaluation of a Resveratrol Dimer, Viniferin, as an HCV Replication Inhibitor
Next Article in Special Issue
Investigating the Cellular Transcriptomic Response Induced by the Makona Variant of Ebola Virus in Differentiated THP-1 Cells
Previous Article in Journal
A New Cell Model for Investigating Prion Strain Selection and Adaptation
Previous Article in Special Issue
Extracellular Vesicles and Ebola Virus: A New Mechanism of Immune Evasion
Article

Cytokine Effects on the Entry of Filovirus Envelope Pseudotyped Virus-Like Particles into Primary Human Macrophages

1
Division of Biotechnology Review and Research 1 (DBRR1), Office of Biotechnology Products (OBP), Center for Drug Evaluation and Research (CDER), US Food and Drug Administration (FDA), Silver Spring, MD 20993, USA
2
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20814, USA
3
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences (USUHS), Bethesda, MD 20814, USA
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(10), 889; https://doi.org/10.3390/v11100889
Received: 17 July 2019 / Revised: 9 September 2019 / Accepted: 18 September 2019 / Published: 23 September 2019
(This article belongs to the Collection Advances in Ebolavirus, Marburgvirus, and Cuevavirus Research)
Macrophages are one of the first and also a major site of filovirus replication and, in addition, are a source of multiple cytokines, presumed to play a critical role in the pathogenesis of the viral infection. Some of these cytokines are known to induce macrophage phenotypic changes in vitro, but how macrophage polarization may affect the cell susceptibility to filovirus entry remains largely unstudied. We generated different macrophage subsets using cytokine pre-treatment and subsequently tested their ability to fuse with beta-lactamase containing virus-like particles (VLP), pseudotyped with the surface glycoprotein of Ebola virus (EBOV) or the glycoproteins of other clinically relevant filovirus species. We found that pre-incubation of primary human monocyte-derived macrophages (MDM) with interleukin-10 (IL-10) significantly enhanced filovirus entry into cells obtained from multiple healthy donors, and the IL-10 effect was preserved in the presence of pro-inflammatory cytokines found to be elevated during EBOV disease. In contrast, fusion of IL-10-treated macrophages with influenza hemagglutinin/neuraminidase pseudotyped VLPs was unchanged or slightly reduced. Importantly, our in vitro data showing enhanced virus entry are consistent with the correlation established between elevated serum IL-10 and increased mortality in filovirus infected patients and also reveal a novel mechanism that may account for the IL-10-mediated increase in filovirus pathogenicity. View Full-Text
Keywords: Ebola virus (EBOV); filoviruses; cytokines; interleukin-10 (IL-10) Ebola virus (EBOV); filoviruses; cytokines; interleukin-10 (IL-10)
Show Figures

Figure 1

MDPI and ACS Style

Stantchev, T.S.; Zack-Taylor, A.; Mattson, N.; Strebel, K.; Broder, C.C.; Clouse, K.A. Cytokine Effects on the Entry of Filovirus Envelope Pseudotyped Virus-Like Particles into Primary Human Macrophages. Viruses 2019, 11, 889. https://doi.org/10.3390/v11100889

AMA Style

Stantchev TS, Zack-Taylor A, Mattson N, Strebel K, Broder CC, Clouse KA. Cytokine Effects on the Entry of Filovirus Envelope Pseudotyped Virus-Like Particles into Primary Human Macrophages. Viruses. 2019; 11(10):889. https://doi.org/10.3390/v11100889

Chicago/Turabian Style

Stantchev, Tzanko S., Autumn Zack-Taylor, Nicholas Mattson, Klaus Strebel, Christopher C. Broder, and Kathleen A. Clouse 2019. "Cytokine Effects on the Entry of Filovirus Envelope Pseudotyped Virus-Like Particles into Primary Human Macrophages" Viruses 11, no. 10: 889. https://doi.org/10.3390/v11100889

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop