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Letter published on 7 March 2019, see Viruses 2019, 11(3), 230.

Open AccessArticle
Viruses 2019, 11(1), 90; https://doi.org/10.3390/v11010090

Double-Stranded RNA High-Throughput Sequencing Reveals a New Cytorhabdovirus in a Bean Golden Mosaic Virus-Resistant Common Bean Transgenic Line

1
Embrapa Recursos Genéticos e Biotecnologia, Brasília 70770-917, Brazil
2
Departamento de Biologia Celular, Universidade de Brasília, Brasília 70910-900, Brazil
3
Embrapa Arroz e Feijão, Goiânia 75375-000, Brazil
4
Departamento de Fitopatologia, Universidade de Brasília, Brasília 70910-900, Brazil
*
Authors to whom correspondence should be addressed.
Received: 31 December 2018 / Revised: 17 January 2019 / Accepted: 18 January 2019 / Published: 21 January 2019
(This article belongs to the Special Issue Plant Virus Ecology and Biodiversity)
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Abstract

Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15–39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean. View Full-Text
Keywords: common bean; Phaseolus vulgaris; rhabdovirus; cytorhabdovirus; dsRNA high throughput sequencing common bean; Phaseolus vulgaris; rhabdovirus; cytorhabdovirus; dsRNA high throughput sequencing
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Alves-Freitas, D.M.T.; Pinheiro-Lima, B.; Faria, J.C.; Lacorte, C.; Ribeiro, S.G.; Melo, F.L. Double-Stranded RNA High-Throughput Sequencing Reveals a New Cytorhabdovirus in a Bean Golden Mosaic Virus-Resistant Common Bean Transgenic Line. Viruses 2019, 11, 90.

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