The Phylogeny and Pathogenesis of Sacbrood Virus (SBV) Infection in European Honey Bees, Apis mellifera
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Bee Sample Collection and SBV Inoculum Preparation
2.3. RNA Extraction
2.4. RT-PCR and RACE
2.5. Phylogenetic Analysis
2.6. Laboratory Induced Sbv Pathogenic Infection
2.7. Cold Stress on Survivorship of SBV-Infected Larvae
3. Results
3.1. Complete Genome Sequences of U.S. Strains of SBV
3.2. Genetic Relationship of SBV Strains Worldwide
3.3. Effect of Cold Stress on Mortality Of SBV-Infected Larvae
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Primer Name | Primer Sequences (5′ to 3′) |
---|---|
F01 | TACGAATCGTGATTCGATTCATT |
F908 | ACGCTAAGTGTGCGCCTAAT |
F1149 | GATATCATCGCGCCTTTGTT |
F1211 | GGAAGTTTGCTAGTATTTACGTG |
F2329 | TGGAGGTAAGGGACAACCTG |
F2408 | TGGACACTGGTGCTAAAGAAGATG |
F3083 | GAAGCTGGGGATGATTTTGA |
F3366 | CGCTATAGGTGGCATGCAGA |
F3619 | GGCATGGATTGATCGAAGTT |
F4526 | CCGGAAATGGCTCATTTAGA |
F4648 | TACCGTAGGAGAGATGTGTTATTGT |
F5394 | TCGAGAAGTGGTTCAGTGCC |
F5436 | AAGTAGTCCAGTGCCCGATG |
F5762 | TCGGATGGTGAAGATGATGA |
F6040 | CCAGCGGTACAAAGAGGAAA |
F6155 | CACTGGATGAGAGCGAATGA |
F6890 | CGGTATTTTATGCGAATGATGT |
F7627 | CCCAGCGTTCTGGAGGAAAT |
F8256 | AACGAGGGCAAACTTGGGAA |
F8471 | CATGGGTTTCATCCCCACGA |
F8598 | GTCGAGCCGCTCTGTATCAA |
F8613 | ATCAAGCGCATGGTCATGGA |
R501 | ACTGCGCGTCTAACATTCCA |
R683 | AACTCTGCTGTGTAGCGTCC |
R793 | TTGTTGCGTTGGTTCGGAAG |
R930 | GTGTTGGGTGCACACTTAGC |
R1250 | AACGGTGACAGCATTTGCAC |
R2450 | CCACAAGCTCCTTGTTGTGA |
R2492 | GAATCCAAAGACTGAAAACCC |
R3435 | CCCGTAACGGTATTCTGCAT |
R3647 | CTGCAGTCCAACTTCGATCA |
R3700 | GCTGCCCAAAAGTTGTAGCC |
R3936 | ACCCCCACCAAATAAGAAGG |
R4515 | TGCATTAAAGCTTGGGGTTC |
R4807 | CAGGTTTAGATGTACACGAGGATG |
R4884 | AACTTCGCAACCAACCAATC |
R5453 | TCGGGCACTGGACTACTTCT |
R5871 | ATTTCCCTCTCTCGCATCAA |
R6256 | CCACTGCCGTCACTAAACCT |
R6300 | TCATTCGCTCTCATCCAGTG |
R6609 | ATACTCCCCACTGCCATCAC |
R6981 | TCCTTAATGGCACGCACATA |
R6995 | TGCATTCCCACAATAGGTCTTT |
R8500 | TAGAATTCCAAGCCAGCGCA |
R8863 | TAAAATGCCATATATTGATATTAATCCA |
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Li, J.; Wang, T.; Evans, J.D.; Rose, R.; Zhao, Y.; Li, Z.; Li, J.; Huang, S.; Heerman, M.; Rodríguez-García, C.; et al. The Phylogeny and Pathogenesis of Sacbrood Virus (SBV) Infection in European Honey Bees, Apis mellifera. Viruses 2019, 11, 61. https://doi.org/10.3390/v11010061
Li J, Wang T, Evans JD, Rose R, Zhao Y, Li Z, Li J, Huang S, Heerman M, Rodríguez-García C, et al. The Phylogeny and Pathogenesis of Sacbrood Virus (SBV) Infection in European Honey Bees, Apis mellifera. Viruses. 2019; 11(1):61. https://doi.org/10.3390/v11010061
Chicago/Turabian StyleLi, Jianghong, Tingyun Wang, Jay D. Evans, Robyn Rose, Yazhou Zhao, Zhiguo Li, Jilian Li, Shaokang Huang, Matthew Heerman, Cristina Rodríguez-García, and et al. 2019. "The Phylogeny and Pathogenesis of Sacbrood Virus (SBV) Infection in European Honey Bees, Apis mellifera" Viruses 11, no. 1: 61. https://doi.org/10.3390/v11010061
APA StyleLi, J., Wang, T., Evans, J. D., Rose, R., Zhao, Y., Li, Z., Li, J., Huang, S., Heerman, M., Rodríguez-García, C., Banmeke, O., Brister, J. R., Hatcher, E. L., Cao, L., Hamilton, M., & Chen, Y. (2019). The Phylogeny and Pathogenesis of Sacbrood Virus (SBV) Infection in European Honey Bees, Apis mellifera. Viruses, 11(1), 61. https://doi.org/10.3390/v11010061