Next Article in Journal
External Quality Assessment (EQA) for Molecular Diagnostics of Zika Virus: Experiences from an International EQA Programme, 2016–2018
Previous Article in Journal
Algal Viruses: The (Atomic) Shape of Things to Come
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Viruses 2018, 10(9), 489; https://doi.org/10.3390/v10090489

Newcastle Disease Virus V Protein Promotes Viral Replication in HeLa Cells through the Activation of MEK/ERK Signaling

College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 2 August 2018 / Revised: 25 August 2018 / Accepted: 5 September 2018 / Published: 12 September 2018
(This article belongs to the Section Animal Viruses)
Full-Text   |   PDF [2190 KB, uploaded 12 September 2018]   |  

Abstract

Newcastle disease virus (NDV) can infect a wide range of domestic and wild bird species. The non-structural V protein of NDV plays an important role in antagonizing innate host defenses to facilitate viral replication. However, there is a lack of knowledge related to the mechanisms through which the V protein regulates viral replication. The extracellular signal-regulated kinase (ERK) signaling pathway in the host is involved in a variety of functions and is activated by several stimuli, including viral replication. In this study, we show that both the lentogenic strain, La Sota, and the velogenic strain, F48E9, of NDV activate the mitogen-activated protein kinase (MEK)/ERK signaling pathway. The pharmacological inhibition of ERK1/2 phosphorylation using the highly selective inhibitors U0126 and SCH772984 resulted in the reduced levels of NDV RNA in cells and virus titers in the cell supernatant, which established an important role for the MEK/ERK signaling pathway in NDV replication. Moreover, the overexpression of the V protein in HeLa cells increased the phosphorylation of ERK1/2 and induced the transcriptional changes in the genes downstream of the MEK/ERK signaling pathway. Taken together, our results demonstrate that the V protein is involved in the ERK signaling pathway-mediated promotion of NDV replication and thus, can be investigated as a potential antiviral target. View Full-Text
Keywords: NDV; V protein; MEK/ERK pathway; p-ERK1/2; antivirals; viral replication; infection NDV; V protein; MEK/ERK pathway; p-ERK1/2; antivirals; viral replication; infection
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Chu, Z.; Ma, J.; Wang, C.; Lu, K.; Li, X.; Liu, H.; Wang, X.; Xiao, S.; Yang, Z. Newcastle Disease Virus V Protein Promotes Viral Replication in HeLa Cells through the Activation of MEK/ERK Signaling. Viruses 2018, 10, 489.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top