Next Article in Journal
Characterization of vB_Kpn_F48, a Newly Discovered Lytic Bacteriophage for Klebsiella pneumoniae of Sequence Type 101
Next Article in Special Issue
The Effect of Aspergillus Thermomutatus Chrysovirus 1 on the Biology of Three Aspergillus Species
Previous Article in Journal
Improved Immune Responses Against Zika Virus After Sequential Dengue and Zika Virus Infection in Humans
Previous Article in Special Issue
Molecular Characterization and Geographic Distribution of a Mymonavirus in the Population of Botrytis cinerea
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessReview
Viruses 2018, 10(9), 481; https://doi.org/10.3390/v10090481

Capsid Structure of dsRNA Fungal Viruses

1
Department of Structure of Macromolecules, Centro Nacional de Biotecnología (CNB-CSIC), Campus Cantoblanco, 28049 Madrid, Spain
2
Centro Nacional de Microbiología/ISCIII, Majadahonda, 28220 Madrid, Spain
3
Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan
4
Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
Current Adress: Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
*
Author to whom correspondence should be addressed.
Received: 21 August 2018 / Revised: 5 September 2018 / Accepted: 5 September 2018 / Published: 7 September 2018
(This article belongs to the Special Issue Mycoviruses)
Full-Text   |   PDF [3754 KB, uploaded 7 September 2018]   |  

Abstract

Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). These capsids show structural variations of the same framework, with 60 asymmetric or symmetric homodimers for ScV-L-A and PsV-F, respectively, monomers with a duplicated similar domain for PcV, and heterodimers of two different proteins for RnQV1. Mycovirus capsid proteins (CP) share a conserved α-helical domain, although the latter may carry different peptides inserted at preferential hotspots. Insertions in the CP outer surface are likely associated with enzymatic activities. Within the capsid, fungal dsRNA viruses show a low degree of genome compaction compared to reoviruses, and contain one to two copies of the RNA-polymerase complex per virion. View Full-Text
Keywords: dsRNA virus; mycovirus; capsid protein; capsid structure; virus evolution; viral lineage; ScV-L-A; PcV; PsV-F; RnQV1 dsRNA virus; mycovirus; capsid protein; capsid structure; virus evolution; viral lineage; ScV-L-A; PcV; PsV-F; RnQV1
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Luque, D.; Mata, C.P.; Suzuki, N.; Ghabrial, S.A.; Castón, J.R. Capsid Structure of dsRNA Fungal Viruses. Viruses 2018, 10, 481.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top