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Capsid Structure of dsRNA Fungal Viruses

Department of Structure of Macromolecules, Centro Nacional de Biotecnología (CNB-CSIC), Campus Cantoblanco, 28049 Madrid, Spain
Centro Nacional de Microbiología/ISCIII, Majadahonda, 28220 Madrid, Spain
Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan
Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
Author to whom correspondence should be addressed.
Current Adress: Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
Viruses 2018, 10(9), 481;
Received: 21 August 2018 / Revised: 5 September 2018 / Accepted: 5 September 2018 / Published: 7 September 2018
(This article belongs to the Special Issue Mycoviruses)
Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). These capsids show structural variations of the same framework, with 60 asymmetric or symmetric homodimers for ScV-L-A and PsV-F, respectively, monomers with a duplicated similar domain for PcV, and heterodimers of two different proteins for RnQV1. Mycovirus capsid proteins (CP) share a conserved α-helical domain, although the latter may carry different peptides inserted at preferential hotspots. Insertions in the CP outer surface are likely associated with enzymatic activities. Within the capsid, fungal dsRNA viruses show a low degree of genome compaction compared to reoviruses, and contain one to two copies of the RNA-polymerase complex per virion. View Full-Text
Keywords: dsRNA virus; mycovirus; capsid protein; capsid structure; virus evolution; viral lineage; ScV-L-A; PcV; PsV-F; RnQV1 dsRNA virus; mycovirus; capsid protein; capsid structure; virus evolution; viral lineage; ScV-L-A; PcV; PsV-F; RnQV1
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MDPI and ACS Style

Luque, D.; Mata, C.P.; Suzuki, N.; Ghabrial, S.A.; Castón, J.R. Capsid Structure of dsRNA Fungal Viruses. Viruses 2018, 10, 481.

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