Next Article in Journal
RNA Phage Biology in a Metagenomic Era
Next Article in Special Issue
A Virus in American Blackcurrant (Ribes americanum) with Distinct Genome Features Reshapes Classification in the Tymovirales
Previous Article in Journal
Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses
Previous Article in Special Issue
Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Viruses 2018, 10(7), 385;

High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics

Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
UMR 1332 Biologie du Fruit et Pathologie, INRA, University of Bordeaux, CS20032, Villenave d’Ornon CEDEX, F-33882 Bordeaux, France
Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Naquera km 4.5, Moncada, 46113 Valencia, Spain
Author to whom correspondence should be addressed.
Received: 23 May 2018 / Revised: 11 July 2018 / Accepted: 19 July 2018 / Published: 21 July 2018
(This article belongs to the Special Issue Fruit Tree Viruses and Viroids)
Full-Text   |   PDF [5846 KB, uploaded 23 July 2018]   |  


Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics. View Full-Text
Keywords: LChV1; Closteroviridae; intra-host variability; high-throughput sequencing; diagnostics LChV1; Closteroviridae; intra-host variability; high-throughput sequencing; diagnostics

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Katsiani, A.; Maliogka, V.I.; Katis, N.; Svanella-Dumas, L.; Olmos, A.; Ruiz-García, A.B.; Marais, A.; Faure, C.; Theil, S.; Lotos, L.; Candresse, T. High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics. Viruses 2018, 10, 385.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top