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Viruses 2018, 10(7), 385; https://doi.org/10.3390/v10070385

High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics

1
Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
2
UMR 1332 Biologie du Fruit et Pathologie, INRA, University of Bordeaux, CS20032, Villenave d’Ornon CEDEX, F-33882 Bordeaux, France
3
Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Naquera km 4.5, Moncada, 46113 Valencia, Spain
*
Author to whom correspondence should be addressed.
Received: 23 May 2018 / Revised: 11 July 2018 / Accepted: 19 July 2018 / Published: 21 July 2018
(This article belongs to the Special Issue Fruit Tree Viruses and Viroids)
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Abstract

Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics. View Full-Text
Keywords: LChV1; Closteroviridae; intra-host variability; high-throughput sequencing; diagnostics LChV1; Closteroviridae; intra-host variability; high-throughput sequencing; diagnostics
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Katsiani, A.; Maliogka, V.I.; Katis, N.; Svanella-Dumas, L.; Olmos, A.; Ruiz-García, A.B.; Marais, A.; Faure, C.; Theil, S.; Lotos, L.; Candresse, T. High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics. Viruses 2018, 10, 385.

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