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Viruses 2018, 10(7), 369; https://doi.org/10.3390/v10070369

Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species

1
Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic
2
Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA
3
Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland
4
Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia
5
Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, 5030 Gembloux, Belgium
6
National Clonal Germplasm Repository, United States Department of Agriculture, Corvallis, OR 97333, USA
*
Author to whom correspondence should be addressed.
Received: 15 June 2018 / Revised: 6 July 2018 / Accepted: 10 July 2018 / Published: 12 July 2018
(This article belongs to the Special Issue Fruit Tree Viruses and Viroids)
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Abstract

Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination. View Full-Text
Keywords: Ribes; currant; closterovirus; recombinants/recombination Ribes; currant; closterovirus; recombinants/recombination
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Koloniuk, I.; Thekke-Veetil, T.; Reynard, J.-S.; Mavrič Pleško, I.; Přibylová, J.; Brodard, J.; Kellenberger, I.; Sarkisova, T.; Špak, J.; Lamovšek, J.; Massart, S.; Ho, T.; Postman, J.D.; Tzanetakis, I.E. Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species. Viruses 2018, 10, 369.

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