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Viruses 2018, 10(7), 369;

Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species

Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic
Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA
Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland
Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia
Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, 5030 Gembloux, Belgium
National Clonal Germplasm Repository, United States Department of Agriculture, Corvallis, OR 97333, USA
Author to whom correspondence should be addressed.
Received: 15 June 2018 / Revised: 6 July 2018 / Accepted: 10 July 2018 / Published: 12 July 2018
(This article belongs to the Special Issue Fruit Tree Viruses and Viroids)
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Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination. View Full-Text
Keywords: Ribes; currant; closterovirus; recombinants/recombination Ribes; currant; closterovirus; recombinants/recombination

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Koloniuk, I.; Thekke-Veetil, T.; Reynard, J.-S.; Mavrič Pleško, I.; Přibylová, J.; Brodard, J.; Kellenberger, I.; Sarkisova, T.; Špak, J.; Lamovšek, J.; Massart, S.; Ho, T.; Postman, J.D.; Tzanetakis, I.E. Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species. Viruses 2018, 10, 369.

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