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Open AccessArticle

The Revisited Genome of Bacillus subtilis Bacteriophage SPP1

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Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
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MICALIS, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France
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Unité de Virologie Moléculaire et Structurale (VMS), CNRS, 91198 Gif-sur-Yvette, France
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Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain
*
Author to whom correspondence should be addressed.
Current address: Folium Science, Unit DX, St Philips Central, Albert road, Bristol BS2 0XJ, UK.
Viruses 2018, 10(12), 705; https://doi.org/10.3390/v10120705
Received: 23 October 2018 / Revised: 6 December 2018 / Accepted: 6 December 2018 / Published: 11 December 2018
(This article belongs to the Special Issue Bacteriophage Genomes and Genomics: News from the Wild)
Bacillus subtilis bacteriophage SPP1 is a lytic siphovirus first described 50 years ago. Its complete DNA sequence was reported in 1997. Here we present an updated annotation of the 44,016 bp SPP1 genome and its correlation to different steps of the viral multiplication process. Five early polycistronic transcriptional units encode phage DNA replication proteins and lysis functions together with less characterized, mostly non-essential, functions. Late transcription drives synthesis of proteins necessary for SPP1 viral particles assembly and for cell lysis, together with a short set of proteins of unknown function. The extensive genetic, biochemical and structural biology studies on the molecular mechanisms of SPP1 DNA replication and phage particle assembly rendered it a model system for tailed phages research. We propose SPP1 as the reference species for a new SPP1-like viruses genus of the Siphoviridae family. View Full-Text
Keywords: Bacteriophage; Siphoviridae; SPP1; Bacillus subtilis; genome organization; DNA replication; virus assembly; virus DNA packaging; virus evolution Bacteriophage; Siphoviridae; SPP1; Bacillus subtilis; genome organization; DNA replication; virus assembly; virus DNA packaging; virus evolution
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Godinho, L.M.; El Sadek Fadel, M.; Monniot, C.; Jakutyte, L.; Auzat, I.; Labarde, A.; Djacem, K.; Oliveira, L.; Carballido-Lopez, R.; Ayora, S.; Tavares, P. The Revisited Genome of Bacillus subtilis Bacteriophage SPP1. Viruses 2018, 10, 705.

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